Cell Biology Antibodies 1

Anti-DES Antibody (CAB0699)

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SKU:
CAB0699
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DES Antibody
Antibody SKU:CAB0699
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 121-470 of human DES (NP_001918.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 121-470 of human DES (NP_001918.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NYIE KVRF LEQQ NAAL AAEV NRLK GREP TRVA ELYE EELR ELRR QVEV LTNQ RARV DVER DNLL DDLQ RLKA KLQE EIQL KEEA ENNL AAFR ADVD AATL ARID LERR IESL NEEI AFLK KVHE EEIR ELQA QLQE QQVQ VEMD MSKP DLTA ALRD IRAQ YETI AAKN ISEA EEWY KSKV SDLT QAAN KNND ALRQ AKQE MMEY RHQI QSYT CEID ALKG TNDS LMRQ MREL EDRF ASEA SGYQ DNIA RLEE EIRH LKDE MARH LREY QDLL NVKM ALDV EIAT YRKL LEGE ESRI NLPI QTYS ALNF RETS PEQR GSEV HTKK TVMI KTIE TRDG EVVS EATQ QQHE VL
Gene ID:1674
Uniprot:P17661
Cellular Location:Cell membrane, Cytoplasm, sarcolemma
Calculated MW:53kDa
Observed MW:54KDa
Synonyms:DES, CSM1, CSM2, LGMD2R, desmin, Desmin
Background:This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
UniProt Protein Function:desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: intermediate filament; fascia adherens; cytosol; neuromuscular junction; Z disc; sarcolemma

Molecular Function:identical protein binding; protein binding; structural constituent of cytoskeleton; cytoskeletal protein binding

Biological Process: muscle contraction; cytoskeleton organization and biogenesis; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1i; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type; Myopathy, Myofibrillar, 1; Muscular Dystrophy, Limb-girdle, Type 2r

NCBI Summary:This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
UniProt Code:P17661
NCBI GenInfo Identifier:6686280
NCBI Gene ID:1674
NCBI Accession:P17661.3
UniProt Secondary Accession:P17661,Q15787, Q549R7, Q549R8, Q549R9, Q8IZR1, Q8IZR6 Q8NES2, Q8NEU6, Q8TAC4, Q8TCX2, Q8TD99,
UniProt Related Accession:P17661
Molecular Weight:
NCBI Full Name:Desmin
NCBI Synonym Full Names:desmin
NCBI Official Symbol:DES  
NCBI Official Synonym Symbols:CSM1; CSM2; LGMD2R  
NCBI Protein Information:desmin; mutant desmin p.K241E; intermediate filament protein
UniProt Protein Name:Desmin
Protein Family:Desmin
UniProt Gene Name:DES  
UniProt Entry Name:DESM_HUMAN
Anti-DES Antibody (CAB0699)Western blot analysis of extracts of various cell lines, using DES antibody (CAB0699) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-DES Antibody (CAB0699)Immunohistochemistry of paraffin-embedded human colon using DES Rabbit pAb (CAB0699) at dilution of 1:100 (40x lens).
Anti-DES Antibody (CAB0699)Immunohistochemistry of paraffin-embedded mouse lung using DES Rabbit pAb (CAB0699) at dilution of 1:100 (40x lens).
Anti-DES Antibody (CAB0699)Immunofluorescence analysis of rat heart using DES Rabbit pAb (CAB0699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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