Immunology Antibodies 2

Anti-DEFA3 Antibody (CAB5340)

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SKU:
CAB5340
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-DEFA3 Antibody
Antibody SKU:CAB5340
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-94 of human DEFA3 (NP_005208.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, 22Rv1, SW480
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-94 of human DEFA3 (NP_005208.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EPLQ ARAD EVAA APEQ IAAD IPEV VVSL AWDE SLAP KHPG SRKN MDCY CRIP ACIA GERR YGTC IYQG RLWA FCC
Gene ID:1668
Uniprot:P59666
Cellular Location:Secreted
Calculated MW:10kDa
Observed MW:13kDa
Synonyms:DEFA3, DEF3, HNP-3, HNP3, HP-3, HP3
Background:Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation.
UniProt Protein Function:defensin, alpha 3: Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus; defense response to virus

NCBI Summary:Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation. [provided by RefSeq, Oct 2014]
UniProt Code:P59666
NCBI GenInfo Identifier:30316323
NCBI Gene ID:1668
NCBI Accession:P59666.1
UniProt Secondary Accession:P59666,P11479, Q14125,
UniProt Related Accession:P59666
Molecular Weight:94
NCBI Full Name:Neutrophil defensin 3
NCBI Synonym Full Names:defensin, alpha 3, neutrophil-specific
NCBI Official Symbol:DEFA3  
NCBI Official Synonym Symbols:HP3; DEF3; HNP3; HP-3; HNP-3  
NCBI Protein Information:neutrophil defensin 3; neutrophil peptide 3; defensin 3, neutrophil-specific
UniProt Protein Name:Neutrophil defensin 3
UniProt Synonym Protein Names:Defensin, alpha 3; HNP-3; HP-3; HP3
Protein Family:Alpha-defensin
UniProt Gene Name:DEFA3  
UniProt Entry Name:DEF3_HUMAN
Anti-DEFA3 Antibody (CAB5340)Western blot analysis of extracts of various cell lines, using DEFA3 antibody (CAB5340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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