Signal Transduction Antibodies 1

Anti-DEF6 Antibody (CAB14337)

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SKU:
CAB14337
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DEF6 Antibody
Antibody SKU:CAB14337
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-330 of human DEF6 (NP_071330.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse brain, Mouse thymus, Rat spleen, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-330 of human DEF6 (NP_071330.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DGPV SSQG YMPY LNKY ILDK VEEG AFVK EHFD ELCW TLTA KKNY RADS NGNS MLSN QDAF RLWC LFNF LSED KYPL IMVP DEVE YLLK KVLS SMSL EVSL GELE ELLA QEAQ VAQT TGGL SVWQ FLEL FNSG RCLR GVGR DTLS MAIH EVYQ ELIQ DVLK QGYL WKRG HLRR NWAE RWFQ LQPS CLCY FGSE ECKE KRGI IPLD AHCC VEVL PDRD GKRC MFCV KTAN RTYE MSAS DTRQ RQEW TAAI QMAI RLQA EGKT SLHK DLKQ KRR
Gene ID:50619
Uniprot:Q9H4E7
Cellular Location:Cell membrane, Cytoplasm, Nucleus
Calculated MW:73kDa
Observed MW:74kDa
Synonyms:DEF6, IBP, SLAT, SWAP70L
Background:
UniProt Protein Function:DEF6: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling.
UniProt Protein Details:

Protein type:GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 6p21.33-p21.1

Cellular Component: membrane

Molecular Function:protein binding

NCBI Summary:DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9H4E7
NCBI GenInfo Identifier:74761430
NCBI Gene ID:50619
NCBI Accession:Q9H4E7.1
UniProt Secondary Accession:Q9H4E7,Q86VF4,
UniProt Related Accession:Q9H4E7
Molecular Weight:73,910 Da
NCBI Full Name:Differentially expressed in FDCP 6 homolog
NCBI Synonym Full Names:DEF6, guanine nucleotide exchange factor
NCBI Official Symbol:DEF6  
NCBI Official Synonym Symbols:IBP; SLAT; SWAP70L  
NCBI Protein Information:differentially expressed in FDCP 6 homolog
UniProt Protein Name:Differentially expressed in FDCP 6 homolog
UniProt Synonym Protein Names:IRF4-binding protein
Protein Family:Differentially expressed in FDCP
UniProt Gene Name:DEF6  
UniProt Entry Name:DEFI6_HUMAN
Anti-DEF6 Antibody (CAB14337)Western blot analysis of extracts of various cell lines, using DEF6 antibody (CAB14337) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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