Cell Biology Antibodies 12

Anti-DDX56 Antibody (CAB9487)

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SKU:
CAB9487
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DDX56 Antibody
Antibody SKU:CAB9487
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 318-547 of human DDX56 (NP_061955.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:HepG2, BxPC-3, NCI-H460, HeLa, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 318-547 of human DDX56 (NP_061955.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EVLG APVK GKRR GRGP KGDK ASDP EAGV ARGI DFHH VSAV LNFD LPPT PEAY IHRA GRTA RANN PGIV LTFV LPTE QFHL GKIE ELLS GENR GPIL LPYQ FRME EIEG FRYR CRDA MRSV TKQA IREA RLKE IKEE LLHS EKLK TYFE DNPR DLQL LRHD LPLH PAVV KPHL GHVP DYLV PPAL RGLV RPHK KRKK LSSS CRKA KRAK SQNP LRSF KHKG KKFR PTAK PS
Gene ID:54606
Uniprot:Q9NY93
Cellular Location:Nucleus, nucleolus
Calculated MW:57kDa/61kDa
Observed MW:61kDa
Synonyms:DDX56, DDX21, DDX26, NOH61
Background:This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:DDX56: May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
UniProt Protein Details:

Protein type:Nucleolus; EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: membrane; nucleolus

Molecular Function:ATP-dependent RNA helicase activity

Biological Process: RNA secondary structure unwinding; rRNA processing

NCBI Summary:This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:Q9NY93
NCBI GenInfo Identifier:20139238
NCBI Gene ID:54606
NCBI Accession:Q9NY93.1
UniProt Secondary Accession:Q9NY93,Q6IAE2, Q9H9I8, A4D2K9, C9JV95,
UniProt Related Accession:Q9NY93
Molecular Weight:57,275 Da
NCBI Full Name:Probable ATP-dependent RNA helicase DDX56
NCBI Synonym Full Names:DEAD-box helicase 56
NCBI Official Symbol:DDX56  
NCBI Official Synonym Symbols:DDX21; DDX26; NOH61  
NCBI Protein Information:probable ATP-dependent RNA helicase DDX56
UniProt Protein Name:Probable ATP-dependent RNA helicase DDX56
UniProt Synonym Protein Names:ATP-dependent 61 kDa nucleolar RNA helicase; DEAD box protein 21; DEAD box protein 56
Protein Family:Probable ATP-dependent RNA helicase
UniProt Gene Name:DDX56  
UniProt Entry Name:DDX56_HUMAN
Anti-DDX56 Polyclonal Antibody (CAB9487)Western blot analysis of extracts of various cell lines, using DDX56 antibody (CAB9487) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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