Anti-DDX50 Antibody (CAB8628)

Antibody Name:	Anti-DDX50 Antibody
Antibody SKU:	CAB8628
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, Jurkat, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPGK LLWG DIME LEAP LEES ESQK KERQ KSDR RKSR HHYD SDEK SETR ENGV TDDL DAPK AKKS KMKE KLNG DTEE GFNR LSDE FSKS HKSR RKDL PNGD IDEY EKKS KRVS SLDT STHK SSDN KLEE TLTR EQK
Gene ID:	79009
Uniprot:	Q9BQ39
Cellular Location:	Nucleus, nucleolus
Calculated MW:	82kDa
Observed MW:	105kDa

Synonyms:

DDX50, GU2, GUB, RH-II/GuB, mcdrh

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

UniProt Protein Function:	DDX50: an RNA helicase of the DEAD-box family.
UniProt Protein Details:	Protein type:EC 3.6.4.13; RNA-binding; Helicase; Nucleolus Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: membrane; nucleolus; plasma membrane Molecular Function:helicase activity; ATP binding Biological Process: metabolic process
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BQ39
NCBI GenInfo Identifier:	13129006
NCBI Gene ID:	79009
NCBI Accession:	NP_076950.1
UniProt Secondary Accession:	Q9BQ39,Q99MJ9,
UniProt Related Accession:	Q9BQ39
Molecular Weight:	82,565 Da
NCBI Full Name:	ATP-dependent RNA helicase DDX50
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
NCBI Official Symbol:	DDX50
NCBI Official Synonym Symbols:	GU2; GUB; mcdrh; RH-II/GuB
NCBI Protein Information:	ATP-dependent RNA helicase DDX50; gu-beta; DEAD box protein 50; nucleolar protein GU2; RNA helicase II/Gu beta; malignant cell derived RNA helicase
UniProt Protein Name:	ATP-dependent RNA helicase DDX50
UniProt Synonym Protein Names:	DEAD box protein 50; Gu-beta; Nucleolar protein Gu2
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DDX50
UniProt Entry Name:	DDX50_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX50 antibody (CAB8628) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (CAB8628) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (CAB8628) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using DDX50 antibody (CAB8628) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (CAB8628) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

DDX50 Antibody (PACO08795)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)