Antibody Name:	Anti-DDX5 Antibody
Antibody SKU:	CAB5296
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_004387.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, SH-SY5Y, HepG2, Jurkat, Mouse lung, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-614 of human DDX5 (NP_004387.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGY SSDR DRGR DRGF GAPR FGGS RAGP LSGK KFGN PGEK LVKK KWNL DELP KFEK NFYQ EHPD LARR TAQE VETY RRSK EITV RGHN CPKP VLNF YEAN FPAN VMDV IARQ NFTE PTAI QAQG WPVA LSGL DMVG VAQT GSGK TLSY LLPA IVHI NHQP FLER GDGP ICLV LAPT RELA QQVQ QVAA EYCR ACRL KSTC IYGG APKG PQIR DLER GVEI CIAT PGRL IDFL ECGK TNLR RTTY LVLD EADR MLDM GFEP QIRK IVDQ IRPD RQTL MWSA TWPK EVRQ LAED FLKD YIHI NIGA LELS ANHN ILQI VDVC HDVE KDEK LIRL MEEI MSEK ENKT IVFV ETKR RCDE LTRK MRRD GWPA MGIH GDKS QQER DWVL NEFK HGKA PILI ATDV ASRG LDVE DVKF VINY DYPN SSED YIHR IGRT ARST KTGT AYTF FTPN NIKQ VSDL ISVL REAN QAIN PKLL QLVE DRGS GRSR GRGG MKDD RRDR YSAG KRGG FNTF RDRE NYDR GYSS LLKR DFGA KTQN GVYS AANY TNGS FGSN FVSA GIQT SFRT GNPT GTYQ NGYD STQQ YGSN VPNM HNGM NQQA YAYP ATAA APMI GYPM PTGY SQ
Gene ID:	1655
Uniprot:	P17844
Cellular Location:	Nucleus, nucleolus
Calculated MW:	60kDa/69kDa
Observed MW:	69kDa

Synonyms:

DDX5, G17P1, HLR1, HUMP68, p68

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Spliceosome; RNA-binding; RNA splicing; EC 3.6.4.13; Nucleolus; Helicase Chromosomal Location of Human Ortholog: 17q21 Cellular Component: nucleoplasm; membrane; nucleolus; ribonucleoprotein complex; nucleus Molecular Function:calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; ATP-dependent RNA helicase activity; RNA helicase activity; ATP binding Biological Process: nuclear mRNA splicing, via spliceosome; circadian rhythm; regulation of osteoblast differentiation; transcription, DNA-dependent; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P17844
NCBI GenInfo Identifier:	129383
NCBI Gene ID:	1655
NCBI Accession:	P17844.1
UniProt Secondary Accession:	P17844,O75681, Q53Y61, B4DLW8, B5BU21, D3DU32, E7ETL9
UniProt Related Accession:	P17844
Molecular Weight:	60,563 Da
NCBI Full Name:	Probable ATP-dependent RNA helicase DDX5
NCBI Synonym Full Names:	DEAD (Asp-Glu-Ala-Asp) box helicase 5
NCBI Official Symbol:	DDX5
NCBI Official Synonym Symbols:	p68; HLR1; G17P1; HUMP68
NCBI Protein Information:	probable ATP-dependent RNA helicase DDX5; DEAD box-5; RNA helicase p68; DEAD box protein 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
UniProt Protein Name:	Probable ATP-dependent RNA helicase DDX5
UniProt Synonym Protein Names:	DEAD box protein 5; RNA helicase p68
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DDX5
UniProt Entry Name:	DDX5_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX5 antibody (CAB5296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of U2OS cells using DDX5 antibody (CAB5296). Blue: DAPI for nuclear staining.