Antibody Name:	DDX39A Rabbit Polyclonal Antibody
Antibody SKU:	CAB7955
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human DDX39A (NP_005795.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, NCI-H460, Jurkat, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human DDX39A (NP_005795.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVRN RSFS LKNV KHFV LDEC DKML EQLD MRRD VQEI FRLT PHEK QCMM FSAT LSKD IRPV CRKF MQDP MEVF VDDE TKLT LHGL QQYY VKLK DSEK NRKL FDLL DVLE FNQV IIFV KSVQ RCMA LAQL LVEQ NFPA IAIH RGMA QEER LSRY QQFK DFQR RILV ATNL FGRG MDIE RVNI VFNY DMPE DSDT YLHR VARA GRFG TKGL AITF VSDE NDAK ILND VQDR FEVN VAEL PEEI DIST YIEQ SR
Gene ID:	10212
Uniprot:	O00148
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	30kDa/36kDa/49kDa
Observed MW:	49kDa

Synonyms:

DDX39A, BAT1, BAT1L, DDX39, DDXL, URH49

Background:

This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

UniProt Protein Function:	DDX39: Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus. Belongs to the DEAD box helicase family. DECD subfamily.
UniProt Protein Details:	Protein type:EC 3.6.4.13; Helicase; RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 19p13.12 Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; spliceosome Molecular Function:ATP-dependent RNA helicase activity; ATPase activity; protein binding Biological Process: mRNA 3'-end processing; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; regulation of gene expression; response to DNA damage stimulus; RNA export from nucleus; RNA secondary structure unwinding; termination of RNA polymerase II transcription
NCBI Summary:	This gene encodes a member of the DEAD box protein family. These proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is thought to play a role in the prognosis of patients with gastrointestinal stromal tumors. A pseudogene of this gene is present on chromosome 13. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]
UniProt Code:	O00148
NCBI GenInfo Identifier:	61212932
NCBI Gene ID:	10212
NCBI Accession:	O00148.2
UniProt Secondary Accession:	O00148,Q8N5M0, Q9BVP6, Q9H5W0, B1Q2N1,
UniProt Related Accession:	O00148
Molecular Weight:	30,488 Da
NCBI Full Name:	ATP-dependent RNA helicase DDX39A
NCBI Synonym Full Names:	DEAD-box helicase 39A
NCBI Official Symbol:	DDX39A
NCBI Official Synonym Symbols:	BAT1; DDXL; BAT1L; DDX39; URH49
NCBI Protein Information:	ATP-dependent RNA helicase DDX39A
UniProt Protein Name:	ATP-dependent RNA helicase DDX39A
UniProt Synonym Protein Names:	DEAD box protein 39; Nuclear RNA helicase URH49
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DDX39A
UniProt Entry Name:	DX39A_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX39A antibody (CAB7955) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse kidney using DDX39A antibody (CAB7955) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using DDX39A antibody (CAB7955) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using DDX39A antibody (CAB7955) at dilution of 1:100 (40x lens).