Epigenetics & Nuclear Signaling Antibodies 2

Anti-DDX20 Antibody (CAB5817)

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SKU:
CAB5817
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DDX20 Antibody
Antibody SKU:CAB5817
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human DDX20 (NP_009135.4).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, ES-2, 293T, HepG2, HeLa, Mouse spleen, Rat testis, Mouse ovary
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human DDX20 (NP_009135.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VQVP APEP TPGP VRIL RTAQ DLSS PRTR TGDV LLAE PADF ESLL LSRP VLEG LRAA GFER PSPV QLKA IPLG RCGL DLIV QAKS GTGK TCVF STIA LDSL VLEN LSTQ ILIL APTR EIAV QIHS VITA IGIK MEGL ECHV FIGG TPLS QDKT RLKK CHIA VGSP GRIK QLIE LDYL NPGS IRLF ILDE ADKL LEEG SFQE QINW IYSS LPAS KQML AVSA TYPE FLAN ALTK YMRD PTFV RLNS SDPS LIGL KQYY
Gene ID:11218
Uniprot:Q9UHI6
Cellular Location:Cytoplasm, Nucleus, gem
Calculated MW:14kDa/92kDa
Observed MW:110kDa
Synonyms:DDX20, DP103, GEMIN3
Background:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.
UniProt Protein Function:GEMIN3: an ubiquitous DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. Interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
UniProt Protein Details:

Protein type:RNA splicing; Nuclear receptor co-regulator; EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 1p21.1-p13.2

Cellular Component: nucleoplasm; cytoskeleton; membrane; transcriptional repressor complex; cytoplasm; SMN complex; nucleus; cytosol

Molecular Function:protein domain specific binding; protein binding; DNA binding; ATP-dependent RNA helicase activity; ATP binding

Biological Process: oogenesis; assembly of spliceosomal tri-snRNP; RNA processing; spliceosomal snRNP biogenesis; positive regulation of apoptosis; regulation of steroid biosynthetic process; gene expression; negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UHI6
NCBI GenInfo Identifier:12643886
NCBI Gene ID:11218
NCBI Accession:Q9UHI6.2
UniProt Secondary Accession:Q9UHI6,Q96F72, Q9NVM3, Q9UF59, Q9UIY0, Q9Y659, B4DWV7
UniProt Related Accession:Q9UHI6
Molecular Weight:824
NCBI Full Name:Probable ATP-dependent RNA helicase DDX20
NCBI Synonym Full Names:DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
NCBI Official Symbol:DDX20  
NCBI Official Synonym Symbols:DP103; GEMIN3  
NCBI Protein Information:probable ATP-dependent RNA helicase DDX20; gemin-3; DEAD box protein 20; component of gems 3; DEAD-box protein DP103; DEAD box protein DP 103; SMN-interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
UniProt Protein Name:Probable ATP-dependent RNA helicase DDX20
UniProt Synonym Protein Names:Component of gems 3; DEAD box protein 20; DEAD box protein DP 103; Gemin-3
Protein Family:Probable ATP-dependent RNA helicase
UniProt Gene Name:DDX20  
UniProt Entry Name:DDX20_HUMAN
Anti-DDX20 Antibody (CAB5817)Western blot analysis of extracts of various cell lines, using DDX20 antibody (CAB5817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-DDX20 Antibody (CAB5817)Immunohistochemistry of paraffin-embedded rat kidney using DDX20 Antibody (CAB5817) at dilution of 1:100 (40x lens).
Anti-DDX20 Antibody (CAB5817)Immunohistochemistry of paraffin-embedded human amygdalitis using DDX20 Antibody (CAB5817) at dilution of 1:100 (40x lens).
Anti-DDX20 Antibody (CAB5817)Immunohistochemistry of paraffin-embedded human colon using DDX20 Antibody (CAB5817) at dilution of 1:100 (40x lens).
Anti-DDX20 Antibody (CAB5817)Immunofluorescence analysis of HeLa cells using DDX20 antibody (CAB5817). Blue: DAPI for nuclear staining.
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