Metabolism Antibodies 3
Anti-DDT Antibody (CAB8816)
- SKU:
- CAB8816
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DDT Antibody |
| Antibody SKU: | CAB8816 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human DDT (NP_001346.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human DDT (NP_001346.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPFL ELDT NLPA NRVP AGLE KRLC AAAA SILG KPAD RVNV TVRP GLAM ALSG STEP CAQL SISS IGVV GTAE DNRS HSAH FFEF LTKE LALG QDRI LIRF FPLE SWQI GKIG TVMT FL |
| Gene ID: | 1652 |
| Uniprot: | P30046 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 11kDa/12kDa |
| Observed MW: | 13kDa |
| Synonyms: | DDT, DDCT |
| Background: | D-dopachrome tautomerase converts D-dopachrome into 5, 6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. |
| UniProt Protein Function: | DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family. |
| UniProt Protein Details: | Protein type:EC 4.1.1.84; Lyase Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cytoplasm Molecular Function:D-dopachrome decarboxylase activity; dopachrome isomerase activity Biological Process: melanin biosynthetic process |
| NCBI Summary: | D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30046 |
| NCBI GenInfo Identifier: | 2828192 |
| NCBI Gene ID: | 1652 |
| NCBI Accession: | P30046.3 |
| UniProt Secondary Accession: | P30046,O00774, O60787, Q13534, B7Z522, |
| UniProt Related Accession: | P30046 |
| Molecular Weight: | 11,323 Da |
| NCBI Full Name: | D-dopachrome decarboxylase |
| NCBI Synonym Full Names: | D-dopachrome tautomerase |
| NCBI Official Symbol: | DDT |
| NCBI Official Synonym Symbols: | DDCT |
| NCBI Protein Information: | D-dopachrome decarboxylase; phenylpyruvate tautomerase II |
| UniProt Protein Name: | D-dopachrome decarboxylase |
| UniProt Synonym Protein Names: | D-dopachrome tautomerase; Phenylpyruvate tautomerase II |
| Protein Family: | D-dopachrome decarboxylase |
| UniProt Gene Name: | DDT |
| UniProt Entry Name: | DOPD_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDT antibody (CAB8816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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