Metabolism Antibodies 3

Anti-DDT Antibody (CAB8816)

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SKU:
CAB8816
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-DDT Antibody
Antibody SKU:CAB8816
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human DDT (NP_001346.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, A-549, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human DDT (NP_001346.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPFL ELDT NLPA NRVP AGLE KRLC AAAA SILG KPAD RVNV TVRP GLAM ALSG STEP CAQL SISS IGVV GTAE DNRS HSAH FFEF LTKE LALG QDRI LIRF FPLE SWQI GKIG TVMT FL
Gene ID:1652
Uniprot:P30046
Cellular Location:Cytoplasm
Calculated MW:11kDa/12kDa
Observed MW:13kDa
Synonyms:DDT, DDCT
Background:D-dopachrome tautomerase converts D-dopachrome into 5, 6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22.
UniProt Protein Function:DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family.
UniProt Protein Details:

Protein type:EC 4.1.1.84; Lyase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytoplasm

Molecular Function:D-dopachrome decarboxylase activity; dopachrome isomerase activity

Biological Process: melanin biosynthetic process

NCBI Summary:D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]
UniProt Code:P30046
NCBI GenInfo Identifier:2828192
NCBI Gene ID:1652
NCBI Accession:P30046.3
UniProt Secondary Accession:P30046,O00774, O60787, Q13534, B7Z522,
UniProt Related Accession:P30046
Molecular Weight:11,323 Da
NCBI Full Name:D-dopachrome decarboxylase
NCBI Synonym Full Names:D-dopachrome tautomerase
NCBI Official Symbol:DDT  
NCBI Official Synonym Symbols:DDCT  
NCBI Protein Information:D-dopachrome decarboxylase; phenylpyruvate tautomerase II
UniProt Protein Name:D-dopachrome decarboxylase
UniProt Synonym Protein Names:D-dopachrome tautomerase; Phenylpyruvate tautomerase II
Protein Family:D-dopachrome decarboxylase
UniProt Gene Name:DDT  
UniProt Entry Name:DOPD_HUMAN
Anti-DDT Polyclonal Antibody (CAB8816)Western blot analysis of extracts of various cell lines, using DDT antibody (CAB8816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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