Cell Biology Antibodies 12
Anti-DDOST Antibody (CAB9056)
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- SKU:
- CAB9056
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DDOST Antibody |
| Antibody SKU: | CAB9056 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human DDOST (NP_005207.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HT-29, HepG2, A-549, Mouse kidney, Mouse liver, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human DDOST (NP_005207.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VCAS GPRT LVLL DNLN VRET HSLF FRSL KDRG FELT FKTA DDPS LSLI KYGE FLYD NLII FSPS VEDF GGNI NVET ISAF IDGG GSVL VAAS SDIG DPLR ELGS ECGI EFDE EKTA VIDH HNYD ISDL GQHT LIVA DTEN LLKA PTIV GKSS LNPI LFRG VGMV ADPD NPLV LDIL TGSS TSYS FFPD KPIT QYPH AVGK NTLL IAGL QARN NARV IFSG SLDF FSDS FFNS AVQK AAPG SQRY SQTG NYEL AVAL SRWV F |
| Gene ID: | 1650 |
| Uniprot: | P39656 |
| Cellular Location: | Endoplasmic reticulum membrane, Single-pass type I membrane protein |
| Calculated MW: | 46kDa/49kDa/50kDa |
| Observed MW: | 54kDa |
| Synonyms: | DDOST, AGER1, CDG1R, GATD6, OKSWcl45, OST, OST48, WBP1 |
| Background: | This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. |
| UniProt Protein Function: | DDOST: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Defects in DDOST are the cause of congenital disorder of glycosylation type 1R (CDG1R). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the DDOST 48 kDa subunit family. |
| UniProt Protein Details: | Protein type:EC 2.4.99.18; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Endoplasmic reticulum; Transferase Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: endoplasmic reticulum membrane; protein complex; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane Molecular Function:protein binding; dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; T cell activation; translation; response to cytokine stimulus; protein amino acid N-linked glycosylation; protein amino acid glycosylation; innate immune response; gene expression; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification Disease: Congenital Disorder Of Glycosylation, Type Ir |
| NCBI Summary: | This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P39656 |
| NCBI GenInfo Identifier: | 239938926 |
| NCBI Gene ID: | 1650 |
| NCBI Accession: | P39656.4 |
| UniProt Secondary Accession: | P39656,O43244, Q5VWA5, Q8NI93, Q9BUI2, B2RDQ4, B4DJE3 B4DLI2, |
| UniProt Related Accession: | P39656 |
| Molecular Weight: | 49,020 Da |
| NCBI Full Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit |
| NCBI Synonym Full Names: | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) |
| NCBI Official Symbol: | DDOST |
| NCBI Official Synonym Symbols: | OST; WBP1; AGER1; CDG1R; OST48; OKSWcl45 |
| NCBI Protein Information: | dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; oligosaccharyltransferase subunit 48; advanced glycation endproduct receptor 1; oligosaccharyltransferase 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit; dolichyl-diphosphooligosaccharide-protein glycotransferase |
| UniProt Protein Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit |
| Protein Family: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase |
| UniProt Gene Name: | DDOST |
| UniProt Entry Name: | OST48_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DDOST antibody (CAB9056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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