Cell Biology Antibodies 6

Anti-DDO Antibody (CAB15338)

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SKU:
CAB15338
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DDO Antibody
Antibody SKU:CAB15338
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DDO (NP_003640.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse heart, Mouse kidney, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human DDO (NP_003640.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRPA RHWE TRFG ARDF GGFQ DCFF RDRL MDTA RIAV VGAG VVGL STAV CISK LVPR CSVT IISD KFTP DTTS DVAA GMLI PHTY PDTP IHTQ KQWF RETF NHLF AIAN SAEA GDAG VHLV SGWQ IFQS TPTE EVPF WADV VLGF RKMT EAEL KKFP QYVF GQAF TTLK CECP AYLP WLEK RIKG SGGW TLTR RIED LWEL HPSF DIVV NCSG LGSR QLAG DSKI FPVR GQVL QVQA PWVE HFIR DGSG LTYI YPGT SHVT LGGT RQKG DWNL SPDA ENSR EILS RCCA LEPS LHGA CNIR EKVG LRPY RPGV RLQT ELLA RDGQ RLPV VHHY GHGS GGIS VHWG TALE AARL VSEC VHAL RTPI PKSN L
Gene ID:8528
Uniprot:Q99489
Cellular Location:Peroxisome
Calculated MW:30kDa/33kDa/37kDa/40kDa
Observed MW:45kDa
Synonyms:DDO, DASOX, DDO-1, DDO-2
Background:The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.
NCBI Summary:The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q99489
NCBI GenInfo Identifier:2494037
NCBI Gene ID:8528
NCBI Accession:Q99489.1
UniProt Secondary Accession:Q99489,Q5JXM4, Q5JXM5, Q5JXM6, Q8N552, A8KAG4,
UniProt Related Accession:Q99489
Molecular Weight:33,980 Da
NCBI Full Name:D-aspartate oxidase
NCBI Synonym Full Names:D-aspartate oxidase
NCBI Official Symbol:DDO  
NCBI Official Synonym Symbols:DASOX; DDO-1; DDO-2  
NCBI Protein Information:D-aspartate oxidase
UniProt Protein Name:D-aspartate oxidase
UniProt Gene Name:DDO  
UniProt Entry Name:OXDD_HUMAN
Anti-DDO Antibody (CAB15338)Western blot analysis of extracts of various cell lines, using DDO antibody (CAB15338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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