Cell Biology Antibodies 17

Anti-DDB1 Antibody (CAB5022)

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SKU:
CAB5022
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
DDBA
Synonyms:
UV-DDB1
Synonyms:
XAP1
Synonyms:
XPCE
Synonyms:
XPE
Synonyms:
XPE-BF

Description

Product Name:DDB1 Rabbit mAb
Product Code:CAB5022
Size:20uL, 50uL, 100uL
Synonyms:DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF
Applications:WB, IHC, IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human DDB1
Applications:WB, IHC, IP
Recommended Dilutions:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, MCF7, NIH/3T3, C6, Mouse brain, Rat lung
Immunogen:A synthesized peptide derived from human DDB1
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1642
Uniprot:Q16531
Cellular Location:Cytoplasm, Nucleus
Calculated MW:127kDa
Observed MW:127KDa
UniProt Protein Function:DDB1: the large subunit of theUV- damaged DNA-binding protein complex (the UV-DDB complex) required for DNA repair. The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of p27kip when associated with CUL4 and SKP2. Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1. DDB1 may recruit specific substrate targeting subunits to the DCX complex. These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Interacts with AMBRA1, ATG16L1, BTRC, DCAF1, DCAF17, DCAF16, DCAF15, DDA1, DET1, DTL, ERCC8, FBXW5, FBXW8, GRWD1, DCAF6, KATNB1, NLE1, NUP43, PAFAH1B1, PHIP, PWP1, RBBP4, RBBP5, RBBP7, RFWD2, SNRNP40, VPRBP, WDR5, WDR5B, WDR12, DCAF4, DCAF5, DCAF11, WDR26, DCAF10, WDR39, DCAF12, WDR42, DCAF8, WDR53, WDR59, WDR61, DCAF7, WSB1, WSB2 and WDTC1. DCX complexes may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. Interacts with NF2, TSC1 and TSC2. Interacts with Simian virus 5 protein V and the HBV X protein. Interaction with SV5 protein V may prevent the recruitment of DCAF proteins to DCX complexes. Interacts with EIF2C1 and EIF2C2. Associates with the E3 ligase complex containing DYRK2, UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Belongs to the DDB1 family.
UniProt Protein Details:

Protein type:DNA repair, damage

Chromosomal Location of Human Ortholog: 11q12-q13

Cellular Component: nucleoplasm; extracellular space; cytoplasm; nucleus

Molecular Function:protein binding; DNA binding; damaged DNA binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of viral genome replication; Wnt receptor signaling pathway; viral reproduction; nucleotide-excision repair; protein ubiquitination during ubiquitin-dependent protein catabolic process; nucleotide-excision repair, DNA damage removal; positive regulation of viral protein levels in host cell; interaction with symbiont; DNA repair; negative regulation of apoptosis

NCBI Summary:The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]
UniProt Code:Q16531
NCBI GenInfo Identifier:12643730
NCBI Gene ID:1642
NCBI Accession:Q16531.1
UniProt Related Accession:Q16531
Molecular Weight:127kDa
NCBI Full Name:DNA damage-binding protein 1
NCBI Synonym Full Names:damage specific DNA binding protein 1
NCBI Official Symbol:DDB1  
NCBI Official Synonym Symbols:XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1  
NCBI Protein Information:DNA damage-binding protein 1
UniProt Protein Name:DNA damage-binding protein 1
UniProt Synonym Protein Names:DDB p127 subunit; DNA damage-binding protein a; DDBa; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; XAP-1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XPE-binding factor; XPE-BF; Xeroderma pigmentosum group E-complementing protein; XPCe
Protein Family:DNA damage-binding protein
UniProt Gene Name:DDB1  
UniProt Entry Name:DDB1_HUMAN
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