Antibody Name:	Anti-DCTN2 Antibody
Antibody SKU:	CAB2200
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human DCTN2 (NP_006391.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Rat
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human DCTN2 (NP_006391.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HIIV NPNA AYDK FKDK RVGT KGLD FSDR IGKT KRTG YESG EYEM LGEG LGVK ETPQ QKYQ RLLH EVQE LTTE VEKI KTTV KESA TEEK LTPV LLAK QLAA LKQQ LVAS HLEK LLGP DAAI NLTD PDGA LAKR LLLQ LEAT KNSK GGSG GKTT GTPP DSSL VTYE LHSR PEQD KFSQ AAKV AELE KRLT ELET AVRC DQDA QNPL SAGL QGAC LMET VELL QAKV SALD LAVL DQVE ARLQ SVLG KVNE IAKH KASV EDAD TQSK VHQL YET
Gene ID:	10540
Uniprot:	Q13561
Cellular Location:	Cytoplasm, Membrane, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:

DCTN2, DCTN50, DYNAMITIN, HEL-S-77, RBP50

Background:

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	dynactin 2: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Motor; Microtubule-binding; Cytoskeletal Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: kinetochore; dynein complex; centrosome; microtubule; dynactin complex; growth cone; membrane; cytoplasm; cytosol; vesicle Molecular Function:protein binding; spectrin binding; motor activity Biological Process: cell proliferation; mitosis; mitotic spindle organization and biogenesis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; G2/M transition of mitotic cell cycle; melanosome transport
NCBI Summary:	This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:	Q13561
NCBI GenInfo Identifier:	22096346
NCBI Gene ID:	10540
NCBI Accession:	Q13561.4
UniProt Secondary Accession:	Q13561,Q86YN2, Q9BW17, B2RBK5,
UniProt Related Accession:	Q13561
Molecular Weight:	401
NCBI Full Name:	Dynactin subunit 2
NCBI Synonym Full Names:	dynactin 2 (p50)
NCBI Official Symbol:	DCTN2
NCBI Official Synonym Symbols:	RBP50; DCTN50; HEL-S-77; DYNAMITIN
NCBI Protein Information:	dynactin subunit 2; p50 dynamitin; dynactin complex 50 kD subunit; dynactin complex 50 kDa subunit; epididymis secretory protein Li 77; 50 kD dynein-associated polypeptide; 50 kDa dynein-associated polypeptide
UniProt Protein Name:	Dynactin subunit 2
UniProt Synonym Protein Names:	50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitin
Protein Family:	Dynactin
UniProt Gene Name:	DCTN2
UniProt Entry Name:	DCTN2_HUMAN

	Western blot analysis of extracts of MCF-7 cells, using DCTN2 antibody (CAB2200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human kidney using DCTN2 antibody (CAB2200) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using DCTN2 antibody (CAB2200) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using DCTN2 antibody (CAB2200) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DCTN2 antibody (CAB2200). Blue: DAPI for nuclear staining.