Cell Biology Antibodies 5
Anti-DCTN2 Antibody (CAB14841)
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- SKU:
- CAB14841
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-DCTN2 Antibody |
| Antibody SKU: | CAB14841 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 280-401 of human DCTN2 (NP_006391.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 280-401 of human DCTN2 (NP_006391.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VLDQ VEAR LQSV LGKV NEIA KHKA SVED ADTQ SKVH QLYE TIQR WSPI ASTL PELV QRLV TIKQ LHEQ AMQF GQLL THLD TTQQ MIAN SLKD NTTL LTQV QTTM RENL ATVE GNFA SIDE RM |
| Gene ID: | 10540 |
| Uniprot: | Q13561 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 44kDa |
| Observed MW: | 44kDa |
| Synonyms: | DCTN2, DCTN50, DYNAMITIN, HEL-S-77, RBP50 |
| Background: | This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | dynactin 2: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Motor; Microtubule-binding; Cytoskeletal Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: kinetochore; dynein complex; centrosome; microtubule; dynactin complex; growth cone; membrane; cytoplasm; cytosol; vesicle Molecular Function:protein binding; spectrin binding; motor activity Biological Process: cell proliferation; mitosis; mitotic spindle organization and biogenesis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; G2/M transition of mitotic cell cycle; melanosome transport |
| NCBI Summary: | This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q13561 |
| NCBI GenInfo Identifier: | 22096346 |
| NCBI Gene ID: | 10540 |
| NCBI Accession: | Q13561.4 |
| UniProt Secondary Accession: | Q13561,Q86YN2, Q9BW17, B2RBK5, |
| UniProt Related Accession: | Q13561 |
| Molecular Weight: | 401 |
| NCBI Full Name: | Dynactin subunit 2 |
| NCBI Synonym Full Names: | dynactin 2 (p50) |
| NCBI Official Symbol: | DCTN2 |
| NCBI Official Synonym Symbols: | RBP50; DCTN50; HEL-S-77; DYNAMITIN |
| NCBI Protein Information: | dynactin subunit 2; p50 dynamitin; dynactin complex 50 kD subunit; dynactin complex 50 kDa subunit; epididymis secretory protein Li 77; 50 kD dynein-associated polypeptide; 50 kDa dynein-associated polypeptide |
| UniProt Protein Name: | Dynactin subunit 2 |
| UniProt Synonym Protein Names: | 50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitin |
| Protein Family: | Dynactin |
| UniProt Gene Name: | DCTN2 |
| UniProt Entry Name: | DCTN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DCTN2 antibody (CAB14841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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