Antibody Name:	Anti-DCK Antibody
Antibody SKU:	CAB1794
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DCK (NP_000779.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, 293T, Mouse liver, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DCK (NP_000779.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATP PKRS CPSF SASS EGTR IKKI SIEG NIAA GKST FVNI LKQL CEDW EVVP EPVA RWCN VQST QDEF EELT MSQK NGGN VLQM MYEK PERW SFTF QTYA CLSR IRAQ LASL NGKL KDAE KPVL FFER SVYS DRYI FASN LYES ECMN ETEW TIYQ DWHD WMNN QFGQ SLEL DGII YLQA TPET CLHR IYLR GRNE EQGI PLEY LEKL HYKH ESWL LHRT LKTN FDYL QEVP ILTL DVNE DFKD KYES LVEK VKEF LSTL
Gene ID:	1633
Uniprot:	P27707
Cellular Location:	Nucleus
Calculated MW:	30kDa
Observed MW:	31kDa

Synonyms:

DCK

Background:

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

UniProt Protein Function:	DCK: Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Belongs to the DCK/DGK family.
UniProt Protein Details:	Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.74; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 4q13.3-q21.1 Cellular Component: cytosol; nucleus Molecular Function:protein homodimerization activity; nucleoside kinase activity; deoxycytidine kinase activity; drug binding; ATP binding Biological Process: pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; phosphorylation; purine salvage; purine base metabolic process; deoxyribonucleoside monophosphate biosynthetic process
NCBI Summary:	Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]
UniProt Code:	P27707
NCBI GenInfo Identifier:	118447
NCBI Gene ID:	1633
NCBI Accession:	P27707.1
UniProt Secondary Accession:	P27707,Q5TZY7, Q6FI11, B2R8V6,
UniProt Related Accession:	P27707
Molecular Weight:	260
NCBI Full Name:	Deoxycytidine kinase
NCBI Synonym Full Names:	deoxycytidine kinase
NCBI Official Symbol:	DCK
NCBI Protein Information:	deoxycytidine kinase; deoxynucleoside kinase
UniProt Protein Name:	Deoxycytidine kinase
Protein Family:	Deoxycytidine kinase
UniProt Gene Name:	DCK
UniProt Entry Name:	DCK_HUMAN

	Western blot analysis of extracts of various cell lines, using DCK Antibody (CAB1794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat kidney using DCK antibody (CAB1794) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using DCK antibody (CAB1794) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using DCK antibody (CAB1794). Blue: DAPI for nuclear staining.