Signal Transduction Antibodies 3

Anti-DCAF7 Antibody (CAB6787)

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SKU:
CAB6787
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DCAF7 Antibody
Antibody SKU:CAB6787
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human DCAF7 (NP_005819.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSLH GKRK EIYK YEAP WTVY AMNW SVRP DKRF RLAL GSFV EEYN NKVQ LVGL DEES SEFI CRNT FDHP YPTT KLMW IPDT KGVY PDLL ATSG DYLR VWRV GETE TRLE CLLN NNKN SDFC APLT SFDW NEVD PYLL GTSS IDTT CTIW GLET GQVL GRVN LVSG HVKT QLIA HDKE VYDI AFSR AGGG RDMF ASVG ADGS VRMF DLRH LEHS TIIY EDPQ HHPL LRLC WNKQ DPNY LATM AMDG MEVV ILDV RVPC TPVA RLNN HRAC VNGI AWAP HSSC HICT AADD HQAL IWDI QQMP RAIE DPIL AYTA EGEI NNVQ WAST QPDW IAIC YNNC LEIL RV
Gene ID:10238
Uniprot:P61962
Cellular Location:Cytoplasm, Nucleus
Calculated MW:16kDa/38kDa
Observed MW:39kDa
Synonyms:DCAF7, AN11, HAN11, SWAN-1, WDR68
Background:This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:WDR68: a WD40 repeat protein. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: cytoplasm; nuclear matrix; nucleoplasm; protein complex

Molecular Function:protein binding

Biological Process: protein ubiquitination

NCBI Summary:This gene encodes a protein with multiple WD40 repeats which facilitate protein-protein interactions and thereby enable the assembly of multiprotein complexes. This protein has been shown to function as a scaffold protein for protein complexes involved in kinase signaling. This highly conserved gene is present in eukaryotic plants, fungi, and animals. The ortholog of this gene was first identified in plants as a key regulator of anthocyanin biosynthesis and flower pigmentation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:P61962
NCBI GenInfo Identifier:48428729
NCBI Gene ID:10238
NCBI Accession:P61962.1
UniProt Secondary Accession:P61962,O15491, Q9DAE4, B4E039, D3DU14,
UniProt Related Accession:P61962
Molecular Weight:16,340 Da
NCBI Full Name:DDB1- and CUL4-associated factor 7
NCBI Synonym Full Names:DDB1 and CUL4 associated factor 7
NCBI Official Symbol:DCAF7  
NCBI Official Synonym Symbols:AN11; HAN11; WDR68; SWAN-1  
NCBI Protein Information:DDB1- and CUL4-associated factor 7
UniProt Protein Name:DDB1- and CUL4-associated factor 7
UniProt Synonym Protein Names:WD repeat-containing protein 68; WD repeat-containing protein An11 homolog
Protein Family:DDB1- and CUL4-associated factor
UniProt Gene Name:DCAF7  
UniProt Entry Name:DCAF7_HUMAN
Anti-DCAF7 Antibody (CAB6787)Western blot analysis of extracts of 293T cells, using DCAF7 antibody (CAB6787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-DCAF7 Antibody (CAB6787)Immunofluorescence analysis of MCF7 cells using DCAF7 antibody (CAB6787). Blue: DAPI for nuclear staining.
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