Signal Transduction Antibodies 2

Anti-DCAF4 Antibody (CAB14869)

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SKU:
CAB14869
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DCAF4 Antibody
Antibody SKU:CAB14869
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DCAF4 (NP_056419.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DCAF4 (NP_056419.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNKS RWQS RRRH GRRS HQQN PWFR LRDS EDRS DSRA AQPA HDSG HGDD ESPS TSSG TAGT SSVP ELPG FYFD PEKK RYFR LLPG HNNC NPLT KESI RQKE MESK RLRL LQEE DRRK KIAR
Gene ID:26094
Uniprot:Q8WV16
Cellular Location:
Calculated MW:44kDa/48kDa/54kDa/55kDa
Observed MW:56kDa
Synonyms:DCAF4, WDR21, WDR21A
Background:This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:WDR21A: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 14q24.3

Biological Process: protein ubiquitination

NCBI Summary:This gene encodes a WD repeat-containing protein that interacts with the Cul4-Ddb1 E3 ligase macromolecular complex. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2009]
UniProt Code:Q8WV16
NCBI GenInfo Identifier:119601489
NCBI Gene ID:26094
NCBI Accession:EAW81083.1
UniProt Secondary Accession:Q8WV16,Q86U31, Q8IV10, Q96K22, Q9Y4P5, B4DUT6, G3V522
UniProt Related Accession:Q8WV16
Molecular Weight:55,694 Da
NCBI Full Name:WD repeat domain 21A, isoform CRA_c
NCBI Synonym Full Names:DDB1 and CUL4 associated factor 4
NCBI Official Symbol:DCAF4  
NCBI Official Synonym Symbols:WDR21; WDR21A  
NCBI Protein Information:DDB1- and CUL4-associated factor 4; WD repeat domain 21A; WD repeat-containing protein 21A
UniProt Protein Name:DDB1- and CUL4-associated factor 4
UniProt Synonym Protein Names:WD repeat-containing protein 21A
Protein Family:DDB1- and CUL4-associated factor
UniProt Gene Name:DCAF4  
UniProt Entry Name:DCAF4_HUMAN
Anti-DCAF4 Antibody (CAB14869)Western blot analysis of extracts of U-251MG cells, using DCAF4 antibody (CAB14869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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