Cell Biology Antibodies 3

Anti-DCAF13 Antibody (CAB13213)

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SKU:
CAB13213
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DCAF13 Antibody
Antibody SKU:CAB13213
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human DCAF13 (NP_056235.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:PC-12, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human DCAF13 (NP_056235.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEEP LHTI LGKT VYTG IDHH WKEA VFAT CGQQ VDIW DEQR TNPI CSMT WGFD SISS VKFN PIET FLLG SCAS DRNI VLYD MRQA TPLK KVIL DMRT NTIC WNPM EAFI FTAA NEDY NLYT FDMR ALDT PVMV HMDH VSAV LDVD YSPT GKEF VSAS FDKS IRIF PVDK SRSR EVYH TKRM QHVI CVKW TSDS KYIM CGSD EMNI RLWK ANAS EKLG VLTS REKA AKDY NQKL KEKF QHYP HIKR IARH RHLP KSIY SQIQ EQRI MKEA RRRK EVNR IKHS KPGS VPLV SEKK KHVV AVVK
Gene ID:25879
Uniprot:Q9NV06
Cellular Location:Nucleus, nucleolus
Calculated MW:10kDa/51kDa
Observed MW:51kDa
Synonyms:DCAF13, GM83, HSPC064, WDSOF1, Sof1
Background:
UniProt Protein Function:WDSOF1: Possible role in ribosomal RNA processing. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Belongs to the WD repeat DCAF13/WDSOF1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleolus; Motility/polarity/chemotaxis; RNA-binding

Chromosomal Location of Human Ortholog: 8q22.3

Cellular Component: cell junction; centrosome; cytoplasm; nucleolus; nucleoplasm; nucleus; small subunit processome

Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); protein ubiquitination; rRNA processing

UniProt Code:Q9NV06
NCBI GenInfo Identifier:160358731
NCBI Gene ID:25879
NCBI Accession:Q9NV06.2
UniProt Secondary Accession:Q9NV06,Q3MII9, Q8NCH8, Q8TC51, Q96JY7, Q9NZX3,
UniProt Related Accession:Q9NV06
Molecular Weight:10,919 Da
NCBI Full Name:DDB1- and CUL4-associated factor 13
NCBI Synonym Full Names:DDB1 and CUL4 associated factor 13
NCBI Official Symbol:DCAF13  
NCBI Official Synonym Symbols:GM83; WDSOF1; HSPC064  
NCBI Protein Information:DDB1- and CUL4-associated factor 13
UniProt Protein Name:DDB1- and CUL4-associated factor 13
UniProt Synonym Protein Names:WD repeat and SOF domain-containing protein 1
Protein Family:DDB1- and CUL4-associated factor
UniProt Gene Name:DCAF13  
UniProt Entry Name:DCA13_HUMAN
Anti-DCAF13 Antibody (CAB13213)Western blot analysis of extracts of various cell lines, using DCAF13 antibody (CAB13213) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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