Signal Transduction Antibodies 2

Anti-DCAF12 Antibody (CAB16510)

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SKU:
CAB16510
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DCAF12 Antibody
Antibody SKU:CAB16510
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human DCAF12 (NP_056212.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:HepG2, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human DCAF12 (NP_056212.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MARK VVSR KRKA PASP GAGS DAQG PQFG WDHS LHKR KRLP PVKR SLVY YLKN REVR LQNE TSYS RVLH GYAA QQLP SLLK EREF H
Gene ID:25853
Uniprot:Q5T6F0
Cellular Location:Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:50kDa
Observed MW:47kDa
Synonyms:DCAF12, CT102, KIAA1892, TCC52, WDR40A
Background:This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates.
UniProt Protein Function:WDR40A: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Belongs to the WD repeat DCAF12 family.
UniProt Protein Details:

Protein type:Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: centrosome; cytoplasm

Biological Process: protein ubiquitination

NCBI Summary:This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. [provided by RefSeq, Jul 2009]
UniProt Code:Q5T6F0
NCBI GenInfo Identifier:74756352
NCBI Gene ID:25853
NCBI Accession:Q5T6F0.1
UniProt Secondary Accession:Q5T6F0,Q5T6E9, Q5T6F1, Q6P3V0, Q7L4F8, Q96PZ5, Q9NXA9 Q9UFJ1, A8KA70, D3DRL6,
UniProt Related Accession:Q5T6F0
Molecular Weight:50,517 Da
NCBI Full Name:DDB1- and CUL4-associated factor 12
NCBI Synonym Full Names:DDB1 and CUL4 associated factor 12
NCBI Official Symbol:DCAF12  
NCBI Official Synonym Symbols:CT102; TCC52; WDR40A; KIAA1892  
NCBI Protein Information:DDB1- and CUL4-associated factor 12
UniProt Protein Name:DDB1- and CUL4-associated factor 12
UniProt Synonym Protein Names:Centrosome-related protein TCC52; Testis cancer centrosome-related protein; WD repeat-containing protein 40A
Protein Family:DDB1- and CUL4-associated factor
UniProt Gene Name:DCAF12  
UniProt Entry Name:DCA12_HUMAN
Anti-DCAF12 Antibody (CAB16510)Western blot analysis of extracts of various cell lines, using DCAF12 antibody (CAB16510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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