KO Validated Antibodies 1

Anti-DBI Antibody (CAB18068)[KO Validated]

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SKU:
CAB18068
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-DBI Antibody [KO Validated]
Antibody SKU:CAB18068
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human DBI (NP_001171513.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human DBI (NP_001171513.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWGD LWLL PPAS ANPG TGTE AEFE KAAE EVRH LKTK PSDE EMLF IYGH YKQA TVGD INTE RPGM LDFT GKAK WDAW NELK GTSK EDAM KAYI NKVE ELKK KYGI
Gene ID:1622
Uniprot:P07108
Cellular Location:Endoplasmic reticulum, Golgi apparatus
Calculated MW:10kDa/11kDa/13kDa/14kDa/16kDa
Observed MW:10kDa
Anti-DBI Antibody [KO Validated] (CAB18068)Western blot analysis of extracts from normal (control) and DBI knockout (KO) 293T cells, using DBI antibody (CAB18068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:DBI, ACBD1, ACBP, CCK-RP, EP
Background:This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:DBI: Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. Belongs to the ACBP family. 6 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Lipid-binding

Chromosomal Location of Human Ortholog: 2q12-q21

Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum

Molecular Function:protein dimerization activity; benzodiazepine receptor binding; lipid binding

Biological Process: learning and/or memory; protein palmitoylation; triacylglycerol metabolic process; behavioral fear response; hair follicle development; transport; lateral ventricle development

UniProt Code:P07108
NCBI GenInfo Identifier:118276
NCBI Gene ID:1622
NCBI Accession:P07108.2
Molecular Weight:12kDa
NCBI Full Name:Acyl-CoA-binding protein
UniProt Protein Name:Acyl-CoA-binding protein
UniProt Synonym Protein Names:Diazepam-binding inhibitor; DBI; Endozepine; EP
Protein Family:DBIRD complex
UniProt Gene Name:DBI  
UniProt Entry Name:ACBP_HUMAN
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