Metabolism Antibodies 2
Anti-DARS Antibody (CAB6574)
- SKU:
- CAB6574
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DARS Antibody |
| Antibody SKU: | CAB6574 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SW480, HepG2, Raji, Mouse liver, Mouse brain, Rat liver, 293T, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KIIS AASE GGAN VFTV SYFK NNAY LAQS PQLY KQMC ICAD FEKV FSIG PVFR AEDS NTHR HLTE FVGL DIEM AFNY HYHE VMEE IADT MVQI FKGL QERF QTEI QTVN KQFP CEPF KFLE PTLR LEYC EALA MLRE AGVE MGDE DDLS TPNE KLLG HLVK EKYD TDFY ILDK YPLA VRPF YTMP DPRN PKQS NSYD MFMR GEEI LSGA QRIH DPQL LTER ALHH GIDL EKIK AYID SFRF GAPP HAGG GIGL ERVT MLFL GLHN VRQT SMFP RDPK RLTP |
| Gene ID: | 1615 |
| Uniprot: | P14868 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 45kDa/57kDa |
| Observed MW: | 57kDa |
| Synonyms: | DARS, HBSL, aspRS |
| Background: | This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family. |
| UniProt Protein Details: | Protein type:Translation; EC 6.1.1.12; Ligase Chromosomal Location of Human Ortholog: 2q21.3 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity Biological Process: tRNA aminoacylation for protein translation; translation; protein complex assembly; gene expression; aspartyl-tRNA aminoacylation Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity |
| NCBI Summary: | This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
| UniProt Code: | P14868 |
| NCBI GenInfo Identifier: | 20178330 |
| NCBI Gene ID: | 1615 |
| NCBI Accession: | P14868.2 |
| UniProt Secondary Accession: | P14868,Q2TNI3, Q32Q69, Q53HV4, Q53YC5, Q68CR9, Q9BW52 A8K3J2, D3DP77, |
| UniProt Related Accession: | P14868 |
| Molecular Weight: | 501 |
| NCBI Full Name: | Aspartate--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | aspartyl-tRNA synthetase |
| NCBI Official Symbol: | DARS |
| NCBI Official Synonym Symbols: | HBSL; aspRS |
| NCBI Protein Information: | aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein |
| UniProt Protein Name: | Aspartate--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein |
| UniProt Gene Name: | DARS |
| UniProt Entry Name: | SYDC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DARS Antibody (CAB6574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using DARS antibody (CAB6574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using DARS antibody (CAB6574) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using DARS antibody (CAB6574) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DARS antibody (CAB6574). Western blot was performed from the immunoprecipitate using DARS antibody (CAB6574) at a dilition of 1:1000. |
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