Metabolism Antibodies 3

Anti-Dapper homolog 3 Antibody (CAB8280)

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SKU:
CAB8280
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:DACT3 Rabbit Polyclonal Antibody
Antibody SKU:CAB8280
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human DACT3 (NP_659493.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, 293T, SH-SY5Y, Mouse heart, Mouse brain, Mouse bone marrow, Mouse liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human DACT3 (NP_659493.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MIRA FSFP VSPE RGRL RGWL EGSL AGLC ELHW LRER QEYR VQQA LRLA QPGM GGAE AEDE EDAD EDED AAAA RRAA AALE EQLE ALPG LVWD LGQQ LGDL SLES GGLE QESG RSSG FYED PSST GGPD SPPS TFCG DSGF SGSS SYGR LGPS EPRG IYAS
Gene ID:147906
Uniprot:Q96B18
Cellular Location:
Calculated MW:64kDa
Observed MW:75kDa
Synonyms:DACT3, DAPPER3, RRR1
Background:
UniProt Protein Function:DACT3: May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. Belongs to the dapper family.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytoplasm

Molecular Function:identical protein binding; protein kinase C binding; protein kinase A binding

Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; negative regulation of cell growth

UniProt Code:Q96B18
NCBI GenInfo Identifier:119368655
NCBI Gene ID:147906
NCBI Accession:Q96B18.2
UniProt Related Accession:Q96B18
Molecular Weight:
NCBI Full Name:Dapper homolog 3
NCBI Synonym Full Names:dishevelled binding antagonist of beta catenin 3
NCBI Official Symbol:DACT3  
NCBI Official Synonym Symbols:RRR1; DAPPER3  
NCBI Protein Information:dapper homolog 3
UniProt Protein Name:Dapper homolog 3
UniProt Synonym Protein Names:Antagonist of beta-catenin Dapper homolog 3; Arginine-rich region 1 protein; Dapper antagonist of catenin 3
Protein Family:Dapper
UniProt Gene Name:DACT3  
UniProt Entry Name:DACT3_HUMAN
Anti-Dapper homolog 3 Polyclonal Antibody (CAB8280)Western blot analysis of extracts of various cell lines, using DACT3 antibody (CAB8280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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