Cell Death Antibodies 2

Anti-DAPK1 Antibody (CAB5741)

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SKU:
CAB5741
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-DAPK1 Antibody
Antibody SKU:CAB5741
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1430 of human DAPK1 (NP_004929.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse lung, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1141-1430 of human DAPK1 (NP_004929.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PCGI FHKV QVNL CRWI HQQS TEGD ADIR LWVN GCKL ANRG AELL VLLV NHGQ GIEV QVRG LETE KIKC CLLL DSVC STIE NVMA TTLP GLLT VKHY LSPQ QLRE HHEP VMIY QPRD FFRA QTLK ETSL TNTM GGYK ESFS SIMC FGCH DVYS QASL GMDI HASD LNLL TRRK LSRL LDPP DPLG KDWC LLAM NLGL PDLV AKYN TSNG APKD FLPS PLHA LLRE WTTY PEST VGTL MSKL RELG RRDA ADFL LKAS SVFK INLD GNGQ EAYA SSCN SGTS YNSI SSVV SR
Gene ID:1612
Uniprot:P53355
Cellular Location:Cytoplasm, cytoskeleton, cytoskeleton
Calculated MW:36kDa/152kDa/160kDa/161kDa
Observed MW:160kDa
Synonyms:DAPK1, DAPK, ROCO3
Background:Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
NCBI Summary:Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Code:P53355
NCBI GenInfo Identifier:317373595
NCBI Gene ID:1612
NCBI Accession:P53355.6
UniProt Secondary Accession:P53355,Q14CQ7, Q1W5W0, Q68CP8, Q6ZRZ3, Q9BTL8, B7ZLD2 B7ZLE7,
UniProt Related Accession:P53355
Molecular Weight:152,467 Da
NCBI Full Name:Death-associated protein kinase 1
NCBI Synonym Full Names:death associated protein kinase 1
NCBI Official Symbol:DAPK1  
NCBI Official Synonym Symbols:DAPK  
NCBI Protein Information:death-associated protein kinase 1
UniProt Protein Name:Death-associated protein kinase 1
Protein Family:Death-associated protein kinase
UniProt Gene Name:DAPK1  
UniProt Entry Name:DAPK1_HUMAN
Anti-DAPK1 Antibody (CAB5741)Western blot analysis of extracts of various cell lines, using DAPK1 Antibody (CAB5741) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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