Cell Death Antibodies 2

Anti-DAP3 Antibody (CAB7003)

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SKU:
CAB7003
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-DAP3 Antibody
Antibody SKU:CAB7003
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 159-398 of human DAP3 (NP_387506.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, Jurkat, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 159-398 of human DAP3 (NP_387506.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HLWV KNCR DLLQ SSYN KQRF DQPL EAST WLKN FKTT NERF LNQI KVQE KYVW NKRE STEK GSPL GEVV EQGI TRVR NATD AVGI VLKE LKRQ SSLG MFHL LVAV DGIN ALWG RTTL KRED KSPI APEE LALV HNLR KMMK NDWH GGAI VSAL SQTG SLFK PRKA YLPQ ELLG KEGF DALD PFIP ILVS NYNP KEFE SCIQ YYLE NNWL QHEK APTE EGKK ELLF LSNA NPSL LERH CAYL
Gene ID:7818
Uniprot:P51398
Cellular Location:Mitochondrion
Calculated MW:41kDa/45kDa
Observed MW:46kDa
Synonyms:DAP3, DAP-3, MRP-S29, MRPS29, bMRP-10, S29mt
Background:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.
UniProt Protein Function:DAP3: Involved in mediating interferon-gamma-induced cell death. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; small ribosomal subunit; mitochondrion; mitochondrial inner membrane; mitochondrial ribosome; nucleolus; nucleus

Molecular Function:protein binding

Biological Process: apoptotic mitochondrial changes; mitochondrial translation; organelle organization and biogenesis

NCBI Summary:Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]
UniProt Code:P51398
NCBI GenInfo Identifier:1706299
NCBI Gene ID:7818
NCBI Accession:P51398.1
UniProt Secondary Accession:P51398,Q13044, Q68CT7, Q96Q20, B4DP59, B4DY62, E7EM60
UniProt Related Accession:P51398
Molecular Weight:398
NCBI Full Name:28S ribosomal protein S29, mitochondrial
NCBI Synonym Full Names:death associated protein 3
NCBI Official Symbol:DAP3  
NCBI Official Synonym Symbols:DAP-3; MRPS29; MRP-S29; bMRP-10  
NCBI Protein Information:28S ribosomal protein S29, mitochondrial; S29mt; death-associated protein 3; mitochondrial 28S ribosomal protein S29; ionizing radiation resistance conferring protein
UniProt Protein Name:28S ribosomal protein S29, mitochondrial
UniProt Synonym Protein Names:Death-associated protein 3; DAP-3; Ionizing radiation resistance conferring protein
Protein Family:28S ribosomal protein
UniProt Gene Name:DAP3  
UniProt Entry Name:RT29_HUMAN
Anti-DAP3 Antibody (CAB7003)Western blot analysis of extracts of various cell lines, using DAP3 Antibody (CAB7003) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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