Cell Death Antibodies 2
Anti-DAP Antibody (CAB6150)
- SKU:
- CAB6150
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-DAP Antibody |
| Antibody SKU: | CAB6150 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human DAP (NP_004385.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, 293T, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human DAP (NP_004385.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSP PEGK LETK AGHP PAVK AGGM RIVQ KHPH TGDT KEEK DKDD QEWE SPSP PKPT VFIS GVIA RGDK DFPP AAAQ VAHQ KPHA SMDK HPSP RTQH IQQP RK |
| Gene ID: | 1611 |
| Uniprot: | P51397 |
| Cellular Location: | |
| Calculated MW: | 11kDa |
| Observed MW: | 17kDa |
| Synonyms: | DAP |
| Background: | This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | DAP: a 15 kDa, proline rich protein that possesses one SH3 binding motif. Negatively regulates autophagy and is involved in mediating interferon-gamma-induced cell death. Localizes in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear. |
| UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 5p15.2 Biological Process: apoptosis; caspase activation; cellular response to amino acid starvation; inhibition of NF-kappaB transcription factor; negative regulation of autophagy; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a basic, proline-rich, 15-kD protein. The protein acts as a positive mediator of programmed cell death that is induced by interferon-gamma. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| UniProt Code: | P51397 |
| NCBI GenInfo Identifier: | 1706298 |
| NCBI Gene ID: | 1611 |
| NCBI Accession: | P51397.3 |
| UniProt Secondary Accession: | P51397,Q6FGC3, Q9BUC9, |
| UniProt Related Accession: | P51397 |
| Molecular Weight: | 11,165 Da |
| NCBI Full Name: | Death-associated protein 1 |
| NCBI Synonym Full Names: | death associated protein |
| NCBI Official Symbol: | DAP |
| NCBI Protein Information: | death-associated protein 1 |
| UniProt Protein Name: | Death-associated protein 1 |
| Protein Family: | Dapper |
| UniProt Gene Name: | DAP |
| UniProt Entry Name: | DAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DAP antibody (CAB6150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using DAP antibody (CAB6150) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using DAP antibody (CAB6150). Blue: DAPI for nuclear staining. |
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