Antibody Name:	Anti-DAO Antibody
Antibody SKU:	CAB5309
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human DAO (NP_001908.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human DAO (NP_001908.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRVV VIGA GVIG LSTA LCIH ERYH SVLQ PLDI KVYA DRFT PLTT TDVA AGLW QPYL SDPN NPQE ADWS QQTF DYLL SHVH SPNA ENLG LFLI SGYN LFHE AIPD PSWK DTVL GFRK LTPR ELDM FPDY GYGW FHTS LILE GKNY LQWL TERL TERG VKFF QRKV ESFE EVAR EGAD VIVN CTGV WAGA LQRD PLLQ PGRG QIMK VDAP WMKH FILT HDPE RGIY NSPY IIPG TQTV TLGG IFQL GNWS ELNN IQDH NTIW EGCC RLEP TLKN ARII GERT GFRP VRPQ IRLE REQL RTGP SNTE VIHN YGHG GYGL TIHW GCAL EAAK LFGR ILEE KKLS RMPP SHL
Gene ID:	1610
Uniprot:	P14920
Cellular Location:	Peroxisome
Calculated MW:	39kDa
Observed MW:	39KDa

Synonyms:

DAO, DAAO, DAMOX, OXDA

Background:

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.

UniProt Protein Function:	DAO: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. Belongs to the DAMOX/DASOX family.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - glycine, serine and threonine; Other Amino Acids Metabolism - D-Arginine and D-ornithine; Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.4.3.3 Chromosomal Location of Human Ortholog: 12q24 Cellular Component: peroxisomal membrane; peroxisomal matrix; mitochondrial outer membrane; peroxisome; cytosol Molecular Function:protein dimerization activity; protein binding; cofactor binding; D-amino-acid oxidase activity; receptor binding Biological Process: proline catabolic process; glyoxylate metabolic process; dopamine biosynthetic process; leucine metabolic process Disease: Schizophrenia
NCBI Summary:	This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]
UniProt Code:	P14920
NCBI GenInfo Identifier:	25453448
NCBI Gene ID:	1610
NCBI Accession:	P14920.3
UniProt Secondary Accession:	P14920,Q16758, Q8N6R2, B2R7I5,
UniProt Related Accession:	P14920
Molecular Weight:	39,474 Da
NCBI Full Name:	D-amino-acid oxidase
NCBI Synonym Full Names:	D-amino-acid oxidase
NCBI Official Symbol:	DAO
NCBI Official Synonym Symbols:	DAAO; OXDA; DAMOX
NCBI Protein Information:	D-amino-acid oxidase; D-amino acid oxidase
UniProt Protein Name:	D-amino-acid oxidase
Protein Family:	D-amino-acid oxidase
UniProt Gene Name:	DAO
UniProt Entry Name:	OXDA_HUMAN

	Western blot analysis of extracts of Rat kidney, using DAO antibody (CAB5309) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Confocal immunofluorescence analysis of L929 cells using DAO Polyclonal Antibody (CAB5309) at dilution of 1:100. Blue: DAPI for nuclear staining.