Immunology Antibodies 1
Anti-DAG1 Antibody (CAB10076)
- SKU:
- CAB10076
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-DAG1 Antibody |
| Antibody SKU: | CAB10076 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IF 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, U2OS, SW480, HeLa, mouse skeletal muscle, mouse lung, mouse heart, rat liver, rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SIVV EWTN NTLP LEPC PKEQ IAGL SRRI AEDD GKPR PAFS NALE PDFK ATSI TVTG SGSC RHLQ FIPV VPPR RVPS EAPP TEVP DRDP EKSS EDDV |
| Gene ID: | 1605 |
| Uniprot: | Q14118 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasm, Nucleus, Secreted, Single-pass type I membrane protein, cytoskeleton, extracellular space, nucleoplasm, postsynaptic cell membrane, sarcolemma, synapse |
| Calculated MW: | 97kDa |
| Observed MW: | 43kDa/95kDa |
| Synonyms: | 156DAG, A3a, AGRNR, DAG, MDDGA9, MDDGC7, MDDGC9, DAG1 |
| Background: | This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. |
| UniProt Protein Function: | DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p21 Cellular Component: dystroglycan complex; extracellular space; costamere; focal adhesion; basolateral plasma membrane; integral to membrane; extracellular region; lipid raft; nucleoplasm; dystrophin-associated glycoprotein complex; postsynaptic membrane; cytoskeleton; cell-cell adherens junction; lamellipodium; cytoplasm; plasma membrane; basement membrane; sarcolemma; filopodium Molecular Function:tubulin binding; viral receptor activity; laminin-1 binding; protein binding; structural constituent of muscle; protein complex binding; SH2 domain binding; calcium ion binding; alpha-actinin binding; actin binding; vinculin binding Biological Process: nerve maturation; response to peptide hormone stimulus; entry of virus into host cell; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; cytoskeletal anchoring; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; NLS-bearing substrate import into nucleus; myelination in the peripheral nervous system; virus-host interaction; morphogenesis of an epithelial sheet; calcium-dependent cell-matrix adhesion; negative regulation of cell migration Disease: Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 9; Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 9 |
| NCBI Summary: | Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010] |
| UniProt Code: | Q14118 |
| NCBI GenInfo Identifier: | 229462879 |
| NCBI Gene ID: | 1605 |
| NCBI Accession: | Q14118.2 |
| UniProt Secondary Accession: | Q14118,Q969J9, A8K6M7, |
| UniProt Related Accession: | Q14118 |
| Molecular Weight: | 97,441 Da |
| NCBI Full Name: | Dystroglycan |
| NCBI Synonym Full Names: | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| NCBI Official Symbol: | DAG1 |
| NCBI Official Synonym Symbols: | A3a; DAG; AGRNR; 156DAG; MDDGC7; MDDGC9 |
| NCBI Protein Information: | dystroglycan |
| UniProt Protein Name: | Dystroglycan |
| UniProt Synonym Protein Names: | Dystrophin-associated glycoprotein 1 |
| Protein Family: | Dystroglycan |
| UniProt Gene Name: | DAG1 |
| UniProt Entry Name: | DAG1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DAG1 antibody (CAB10076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of H9C2 cells using DAG1 Rabbit pAb (CAB10076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using DAG1 Rabbit pAb (CAB10076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using DAG1 Rabbit pAb (CAB10076) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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