Name: Anti-DAD1 Antibody (CAB14723)
Brand: Cell Death Antibodies 1
SKU: CAB14723
Availability: OutOfStock

Antibody Name:	Anti-DAD1 Antibody
Antibody SKU:	CAB14723
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAD1 (NP_001335.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, Mouse thymus, Mouse brain, Mouse kidney, Mouse liver, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAD1 (NP_001335.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAS VVSV ISRF LEEY LSST PQRL KLLD AYLL YILL TGAL QFGY CLLV GTFP FNSF LSGF ISCV GSFI LAVC LRIQ INPQ NKAD FQGI SPER AFAD FLFA
Gene ID:	1603
Uniprot:	P61803
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	12kDa
Observed MW:	22kDa

Synonyms:

DAD1, OST2

Background:

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.

UniProt Protein Function:	DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.99.18; Transferase; Membrane protein, multi-pass; Membrane protein, integral; Apoptosis Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complex; integral to membrane Molecular Function:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity Biological Process: response to drug; blastocyst development; cellular protein metabolic process; apoptosis; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to nutrient; negative regulation of apoptosis
NCBI Summary:	DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
UniProt Code:	P61803
NCBI GenInfo Identifier:	48428858
NCBI Gene ID:	1603
NCBI Accession:	P61803.3
UniProt Secondary Accession:	P61803,O08552, O70364, P46966, P46968, Q6FGA3, Q96GB7 D3DS25,
UniProt Related Accession:	P61803
Molecular Weight:	12,497 Da
NCBI Full Name:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
NCBI Synonym Full Names:	defender against cell death 1
NCBI Official Symbol:	DAD1
NCBI Official Synonym Symbols:	OST2
NCBI Protein Information:	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic)
UniProt Protein Name:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Synonym Protein Names:	Defender against cell death 1; DAD-1
Protein Family:	DASH complex
UniProt Gene Name:	DAD1
UniProt Entry Name:	DAD1_HUMAN

Western blot analysis of extracts of various cell lines, using DAD1 antibody (CAB14723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.

Antibodies

DAD1 Antibody (PACO08735)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)