Epigenetics & Nuclear Signaling Antibodies 4

Anti-DACH1 Antibody (CAB6895)

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SKU:
CAB6895
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-DACH1 Antibody
Antibody SKU:CAB6895
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-570 of human DACH1 (NP_542937.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse kidney, Mouse lung, Mouse heart, Rat kidney, Rat heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-570 of human DACH1 (NP_542937.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EGRR PGSH PSSH RSSS VSSS PART ESSS DRIP VHQN GLSM NQML MGLS PNVL PGPK EGDL AGHD MGHE SKRM HIEK DETP LSTP TARD SLDK LSLT GHGQ PLPP GFPS PFLF PDGL SSIE T
Gene ID:1602
Uniprot:Q9UI36
Cellular Location:Nucleus
Calculated MW:52kDa/57kDa/73kDa/78kDa
Observed MW:90kDa
Synonyms:DACH1, DACH
Background:This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:DACH1: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin- dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain. Belongs to the DACH/dachshund family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 13q22

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; cytoplasm; nucleolus; nucleus

Molecular Function:protein binding; DNA binding

Biological Process: cell proliferation; development of primary female sexual characteristics; suckling behavior; negative regulation of fibroblast proliferation; respiratory gaseous exchange; negative regulation of transcription from RNA polymerase II promoter; transcriptional preinitiation complex assembly; negative regulation of cell migration

UniProt Code:Q9UI36
NCBI GenInfo Identifier:519668677
NCBI Gene ID:
NCBI Accession:Q9UI36.3
Molecular Weight:
NCBI Full Name:Dachshund homolog 1
UniProt Protein Name:Dachshund homolog 1
Protein Family:Dachshund
UniProt Gene Name:DACH1  
UniProt Entry Name:DACH1_HUMAN
Anti-DACH1 Antibody (CAB6895)Western blot analysis of extracts of various cell lines, using DACH1 antibody (CAB6895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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