Name: Anti-DAAM2 Antibody (CAB7463)
Brand: Metabolism Antibodies 2
SKU: CAB7463
Availability: OutOfStock

Antibody Name:	Anti-DAAM2 Antibody
Antibody SKU:	CAB7463
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human DAAM2 (NP_056160.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A375, Mouse brain, Mouse testis, Mouse kidney, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human DAAM2 (NP_056160.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPR KRSH HGLG FLCC FGGS DIPE INLR DNHP LQFM EFSS PIPN AEEL NIRF AELV DELD LTDK NREA MFAL PPEK KWQI YCSK KK
Gene ID:	23500
Uniprot:	Q86T65
Cellular Location:
Calculated MW:	123kDa
Observed MW:	123kDa

Synonyms:	DAAM2, dJ90A20A.1
Background:

UniProt Protein Function:	DAAM2: Belongs to the formin homology family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 6p21.2
UniProt Code:	Q86T65
NCBI GenInfo Identifier:	62906888
NCBI Gene ID:	23500
NCBI Accession:	Q86T65.3
UniProt Secondary Accession:	Q86T65,Q5T4T8, Q5T4U0, Q9NQI5, Q9Y4G0, G5EA45,
UniProt Related Accession:	Q86T65
Molecular Weight:	123,370 Da
NCBI Full Name:	Disheveled-associated activator of morphogenesis 2
NCBI Synonym Full Names:	dishevelled associated activator of morphogenesis 2
NCBI Official Symbol:	DAAM2
NCBI Official Synonym Symbols:	dJ90A20A.1
NCBI Protein Information:	disheveled-associated activator of morphogenesis 2
UniProt Protein Name:	Disheveled-associated activator of morphogenesis 2
Protein Family:	Disheveled-associated activator of morphogenesis
UniProt Gene Name:	DAAM2
UniProt Entry Name:	DAAM2_HUMAN

	Western blot analysis of extracts of various cell lines, using DAAM2 antibody (CAB7463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using DAAM2 Antibody (CAB7463) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using DAAM2 Rabbit pAb (CAB7463) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using DAAM2 Rabbit pAb (CAB7463) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of H9C2 cells using DAAM2 Rabbit pAb (CAB7463) at dilution of 1:100. Blue: DAPI for nuclear staining.