Metabolism Antibodies 2
Anti-DAAM2 Antibody (CAB7463)
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- SKU:
- CAB7463
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-DAAM2 Antibody |
| Antibody SKU: | CAB7463 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human DAAM2 (NP_056160.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A375, Mouse brain, Mouse testis, Mouse kidney, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human DAAM2 (NP_056160.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPR KRSH HGLG FLCC FGGS DIPE INLR DNHP LQFM EFSS PIPN AEEL NIRF AELV DELD LTDK NREA MFAL PPEK KWQI YCSK KK |
| Gene ID: | 23500 |
| Uniprot: | Q86T65 |
| Cellular Location: | |
| Calculated MW: | 123kDa |
| Observed MW: | 123kDa |
| Synonyms: | DAAM2, dJ90A20A.1 |
| Background: |
| UniProt Protein Function: | DAAM2: Belongs to the formin homology family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 6p21.2 |
| UniProt Code: | Q86T65 |
| NCBI GenInfo Identifier: | 62906888 |
| NCBI Gene ID: | 23500 |
| NCBI Accession: | Q86T65.3 |
| UniProt Secondary Accession: | Q86T65,Q5T4T8, Q5T4U0, Q9NQI5, Q9Y4G0, G5EA45, |
| UniProt Related Accession: | Q86T65 |
| Molecular Weight: | 123,370 Da |
| NCBI Full Name: | Disheveled-associated activator of morphogenesis 2 |
| NCBI Synonym Full Names: | dishevelled associated activator of morphogenesis 2 |
| NCBI Official Symbol: | DAAM2 |
| NCBI Official Synonym Symbols: | dJ90A20A.1 |
| NCBI Protein Information: | disheveled-associated activator of morphogenesis 2 |
| UniProt Protein Name: | Disheveled-associated activator of morphogenesis 2 |
| Protein Family: | Disheveled-associated activator of morphogenesis |
| UniProt Gene Name: | DAAM2 |
| UniProt Entry Name: | DAAM2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DAAM2 antibody (CAB7463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using DAAM2 Antibody (CAB7463) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using DAAM2 Rabbit pAb (CAB7463) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using DAAM2 Rabbit pAb (CAB7463) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of H9C2 cells using DAAM2 Rabbit pAb (CAB7463) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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