Metabolism Antibodies 1

Anti-DAAM1 Antibody (CAB12929)

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SKU:
CAB12929
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-DAAM1 Antibody
Antibody SKU:CAB12929
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 769-1068 of human DAAM1 (NP_001257449.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HepG2, Mouse heart, Mouse brain, Mouse liver, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 769-1068 of human DAAM1 (NP_001257449.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FKKK FAER VAEV KPKV EAIR SGSE EVFR SGAL KQLL EVVL AFGN YMNK GQRG NAYG FKIS SLNK IADT KSSI DKNI TLLH YLIT IVEN KYPS VLNL NEEL RDIP QAAK VNMT ELDK EIST LRSG LKAV ETEL EYQK SQPP QPGD KFVS VVSQ FITV ASFS FSDV EDLL AEAK DLFT KAVK HFGE EAGK IQPD EFFG IFDQ FLQA VSEA KQEN ENMR KKKE EEER RARM EAQL KEQR ERER KMRK AKEN SEES GEFD DLVS ALRS GEVF DKDL SKLK RNRK RITN QMTD SSRE RPIT KLNF
Gene ID:23002
Uniprot:Q9Y4D1
Cellular Location:Cytoplasm
Calculated MW:100kDa/122kDa/123kDa
Observed MW:123kDa
Synonyms:DAAM1
Background:Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins.
UniProt Protein Function:DAAM1: Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: cytoplasm; cytosol; membrane; plasma membrane; stress fiber

Molecular Function:identical protein binding; protein binding

Biological Process: Wnt receptor signaling pathway, planar cell polarity pathway

NCBI Summary:Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]
UniProt Code:Q9Y4D1
NCBI GenInfo Identifier:34098767
NCBI Gene ID:23002
NCBI Accession:Q9Y4D1.2
UniProt Secondary Accession:Q9Y4D1,Q86U34, Q8N1Z8, Q8TB39,
UniProt Related Accession:Q9Y4D1
Molecular Weight:100,745 Da
NCBI Full Name:Disheveled-associated activator of morphogenesis 1
NCBI Synonym Full Names:dishevelled associated activator of morphogenesis 1
NCBI Official Symbol:DAAM1  
NCBI Protein Information:disheveled-associated activator of morphogenesis 1
UniProt Protein Name:Disheveled-associated activator of morphogenesis 1
Protein Family:Disheveled-associated activator of morphogenesis
UniProt Gene Name:DAAM1  
UniProt Entry Name:DAAM1_HUMAN
Anti-DAAM1 Antibody (CAB12929)Western blot analysis of extracts of various cell lines, using DAAM1 antibody (CAB12929) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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