Cell Cycle Antibodies 1
Anti-Cytokeratin-18 Antibody (CAB1022)
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- SKU:
- CAB1022
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-Cytokeratin-18 Antibody |
| Antibody SKU: | CAB1022 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human Cytokeratin-18 (NP_000215.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, HepG2, HeLa, Mouse liver, Mouse pancreas, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human Cytokeratin-18 (NP_000215.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SHYF KIIE DLRA QIFA NTVD NARI VLQI DNAR LAAD DFRV KYET ELAM RQSV ENDI HGLR KVID DTNI TRLQ LETE IEAL KEEL LFMK KNHE EEVK GLQA QIAS SGLT VEVD APKS QDLA KIMA DIRA QYDE LARK NREE LDKY WSQQ IEES TTVV TTQS AEVG AAET TLTE LRRT VQSL EIDL DSMR NLKA SLEN SLRE VEAR YALQ MEQL NGIL LHLE SELA QTRA EGQR QAQE YEAL LNIK VKLE AEIA TYRR LLED GEDF NLGD ALDS SNSM QTIQ KTTT RRIV DGKV VSET NDTK VLRH |
| Gene ID: | 3875 |
| Uniprot: | P05783 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus, perinuclear region |
| Calculated MW: | 48kDa |
| Observed MW: | 48kDa |
| Synonyms: | KRT18, CK-18, CYK18, K18, keratin 18 |
| Background: | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis. |
| UniProt Protein Details: | Protein type:Nucleolus; Cytoskeletal Chromosomal Location of Human Ortholog: 12q13 Cellular Component: cytoplasm; intermediate filament; keratin filament; microtubule organizing center; nucleolus; perinuclear region of cytoplasm Molecular Function:protein binding; structural molecule activity Biological Process: anatomical structure morphogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; viral reproduction Disease: Cirrhosis, Familial |
| NCBI Summary: | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05783 |
| NCBI GenInfo Identifier: | 125083 |
| NCBI Gene ID: | 3875 |
| NCBI Accession: | P05783.2 |
| UniProt Secondary Accession: | P05783,Q53G38, Q5U0N8, Q9BW26, |
| UniProt Related Accession: | P05783 |
| Molecular Weight: | |
| NCBI Full Name: | Keratin, type I cytoskeletal 18 |
| NCBI Synonym Full Names: | keratin 18 |
| NCBI Official Symbol: | KRT18 |
| NCBI Official Synonym Symbols: | K18; CK-18; CYK18 |
| NCBI Protein Information: | keratin, type I cytoskeletal 18 |
| UniProt Protein Name: | Keratin, type I cytoskeletal 18 |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18 |
| Protein Family: | Keratin |
| UniProt Gene Name: | KRT18 |
| UniProt Entry Name: | K1C18_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Cytokeratin-18 antibody (CAB1022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using Cytokeratin-18 antibody (CAB1022) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human small intestine using Cytokeratin-18 antibody (CAB1022) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using Cytokeratin-18 antibody (CAB1022) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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