Antibody Name:	Anti-CYTH2 Antibody
Antibody SKU:	CAB7035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 150-350 of human CYTH2 (NP_059431.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, HepG2, Mouse ovary, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 150-350 of human CYTH2 (NP_059431.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SFRL PGEA QKID RMME AFAQ RYCL CNPG VFQS TDTC YVLS FAVI MLNT SLHN PNVR DKPG LERF VAMN RGIN EGGD LPEE LLRN LYDS IRNE PFKI PEDD GNDL THTF FNPD REGW LLKL GGGR VKTW KRRW FILT DNCL YYFE YTTD KEPR GIIP LENL SIRE VDDP RKPN CFEL YIPN NKGQ LIKA CKTE ADGR VVEG N
Gene ID:	9266
Uniprot:	Q99418
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Peripheral membrane protein, growth cone
Calculated MW:	46kDa
Observed MW:	47kDa

Synonyms:

CYTH2, ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like

Background:

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	cytohesin 2: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GEFs; GEFs, ARF Chromosomal Location of Human Ortholog: 19q13.33 Cellular Component: membrane; cytoplasm; plasma membrane; trans-Golgi network Molecular Function:protein binding; lipid binding; ARF guanyl-nucleotide exchange factor activity Biological Process: regulation of cell adhesion; vesicle-mediated transport; endocytosis; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity
NCBI Summary:	The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	Q99418
NCBI GenInfo Identifier:	13124707
NCBI Gene ID:	9266
NCBI Accession:	Q99418.2
UniProt Related Accession:	Q99418
Molecular Weight:	46kDa
NCBI Full Name:	Cytohesin-2
NCBI Synonym Full Names:	cytohesin 2
NCBI Official Symbol:	CYTH2
NCBI Official Synonym Symbols:	ARNO; CTS18; PSCD2; SEC7L; PSCD2L; CTS18.1; Sec7p-L; Sec7p-like; cytohesin-2
NCBI Protein Information:	cytohesin-2
UniProt Protein Name:	Cytohesin-2
UniProt Synonym Protein Names:	ARF exchange factor; ARF nucleotide-binding site opener; Protein ARNO; PH, SEC7 and coiled-coil domain-containing protein 2
Protein Family:	Cytohesin
UniProt Gene Name:	CYTH2
UniProt Entry Name:	CYH2_HUMAN