Cell Biology Antibodies 6

Anti-CYTH1 Antibody (CAB15351)

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SKU:
CAB15351
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CYTH1 Antibody
Antibody SKU:CAB15351
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH1 (NP_004753.1).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, Mouse brain, Mouse heart, Mouse spleen, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH1 (NP_004753.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEED DSYV PSDL TAEE RQEL ENIR RRKQ ELLA DIQR LKDE IAEV ANEI ENLG STEE RKNM QRNK QVAM GR
Gene ID:9267
Uniprot:Q15438
Cellular Location:Cell membrane, Cytoplasm, Peripheral membrane protein, cytosol
Calculated MW:39kDa/46kDa
Observed MW:46kDa
Synonyms:CYTH1, B2-1, CYTOHESIN-1, D17S811E, PSCD1, SEC7
Background:The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:cytohesin 1: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; GEFs; GEFs, ARF

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extrinsic to internal side of plasma membrane; tight junction; cytoplasm; plasma membrane; trans-Golgi network; cytosol

Molecular Function:protein binding; lipid binding; ARF guanyl-nucleotide exchange factor activity

Biological Process: vesicle-mediated transport; regulation of cell adhesion; regulation of ARF protein signal transduction; positive regulation of GTPase activity

NCBI Summary:The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:Q15438
NCBI GenInfo Identifier:2498175
NCBI Gene ID:9267
NCBI Accession:Q15438.1
UniProt Secondary Accession:Q15438,Q9P123, Q9P124, A6NFW7, B7Z1T4,
UniProt Related Accession:Q15438
Molecular Weight:39,470 Da
NCBI Full Name:Cytohesin-1
NCBI Synonym Full Names:cytohesin 1
NCBI Official Symbol:CYTH1  
NCBI Official Synonym Symbols:B2-1; SEC7; PSCD1; D17S811E; CYTOHESIN-1  
NCBI Protein Information:cytohesin-1; PH, SEC7 and coiled-coil domain-containing protein 1; SEC7 homolog B2-1; cytoadhesin 1; homolog of secretory protein SEC7; pleckstrin homology, Sec7 and coiled-coil domains 1
UniProt Protein Name:Cytohesin-1
UniProt Synonym Protein Names:PH, SEC7 and coiled-coil domain-containing protein 1; SEC7 homolog B2-1
Protein Family:Cytohesin
UniProt Gene Name:CYTH1  
UniProt Entry Name:CYH1_HUMAN
Anti-CYTH1 Antibody (CAB15351)Western blot analysis of extracts of various cell lines, using CYTH1 antibody (CAB15351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-CYTH1 Antibody (CAB15351)Immunofluorescence analysis of L929 cells using CYTH1 antibody (CAB15351) at dilution of 1:100. Blue: DAPI for nuclear staining.
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