Metabolism Antibodies 2

Anti-CYP7B1 Antibody (CAB17872)

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SKU:
CAB17872
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-CYP7B1 Antibody
Antibody SKU:CAB17872
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CYP7B1 (NP_004811.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse, Rat
Positive Samples:mouse kidney, rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CYP7B1 (NP_004811.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Isotype:IgG
Sequence:VIVC DNNK FISE LRDD FLKF DDKF AYLV SNIP IELL GNVK SIRE KIIK CFSS EKLA KMQG WSEV FQSR QDVL EKYY VHED LEIG AHHL GFLW ASVA NTIP T
Gene ID:9420
Uniprot:O75881
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:58kDa
Observed MW:58kDa
Synonyms:CYP7B1, CBAS3, CP7B, SPG5A
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.
UniProt Protein Function:CYP7B1: Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3). Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:EC 1.14.13.100; Oxidoreductase; Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: endoplasmic reticulum membrane

Molecular Function:oxysterol 7-alpha-hydroxylase activity

Biological Process: bile acid biosynthetic process; sterol metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 3; Spastic Paraplegia 5a, Autosomal Recessive

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]
UniProt Code:O75881
NCBI GenInfo Identifier:20141320
NCBI Gene ID:9420
NCBI Accession:O75881.2
UniProt Secondary Accession:O75881,Q9UNF5, B2RN07,
UniProt Related Accession:O75881
Molecular Weight:58,256 Da
NCBI Full Name:25-hydroxycholesterol 7-alpha-hydroxylase
NCBI Synonym Full Names:cytochrome P450 family 7 subfamily B member 1
NCBI Official Symbol:CYP7B1  
NCBI Official Synonym Symbols:CP7B; CBAS3; SPG5A  
NCBI Protein Information:25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Protein Name:25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Synonym Protein Names:Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase
Protein Family:25-hydroxycholesterol 7-alpha-hydroxylase
UniProt Gene Name:CYP7B1  
UniProt Entry Name:CP7B1_HUMAN
Anti-CYP7B1 Antibody (CAB17872)Western blot analysis of extracts of various cells, using CYP7B1 antibody (CAB17872) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
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