Metabolism Antibodies 1
Anti-CYP7A1 Antibody (CAB10615)
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- SKU:
- CAB10615
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CYP7A1 Antibody |
| Antibody SKU: | CAB10615 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human CYP7A1 (NP_000771.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human CYP7A1 (NP_000771.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LILG IRRR QTGE PPLE NGLI PYLG CALQ FGAN PLEF LRAN QRKH GHVF TCKL MGKY VHFI TNPL SYHK VLCH GKYF DWKK FHFA TSAK AFGH RSID PMDG NTTE NIND TFIK TLQG HALN SLTE SMME NLQR IMRP PVSS NSKT AAWV TEGM YSFC YRVM FEAG YLTI FGRD LTRR DTQK AHIL NNLD NFKQ FDKV FPAL VAGL PIHM FRTA HNAR EKLA ESLR HENL QKRE SISE LISL RMFL NDTL STFD DLEK AKTH L |
| Gene ID: | 1581 |
| Uniprot: | P22680 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein |
| Calculated MW: | 57kDa |
| Observed MW: | 50kDa |
| Synonyms: | CYP7A1, CP7A, CYP7, CYPVII |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. |
| UniProt Protein Function: | CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:EC 1.14.13.17; Lipid Metabolism - primary bile acid biosynthesis; Endoplasmic reticulum; Oxidoreductase Chromosomal Location of Human Ortholog: 8q11-q12 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle Molecular Function:cholesterol 7-alpha-monooxygenase activity Biological Process: bile acid biosynthetic process; cholesterol catabolic process; cholesterol homeostasis; sterol metabolic process |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010] |
| UniProt Code: | P22680 |
| NCBI GenInfo Identifier: | 544084 |
| NCBI Gene ID: | 1581 |
| NCBI Accession: | P22680.2 |
| UniProt Secondary Accession: | P22680,P78454, Q3MIL8, Q7KZ19, |
| UniProt Related Accession: | P22680 |
| Molecular Weight: | 57,661 Da |
| NCBI Full Name: | Cholesterol 7-alpha-monooxygenase |
| NCBI Synonym Full Names: | cytochrome P450 family 7 subfamily A member 1 |
| NCBI Official Symbol: | CYP7A1 |
| NCBI Official Synonym Symbols: | CP7A; CYP7; CYPVII |
| NCBI Protein Information: | cholesterol 7-alpha-monooxygenase |
| UniProt Protein Name: | Cholesterol 7-alpha-monooxygenase |
| UniProt Synonym Protein Names: | CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1 |
| Protein Family: | Cholesterol 7-alpha-monooxygenase |
| UniProt Gene Name: | CYP7A1 |
| UniProt Entry Name: | CP7A1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP7A1 antibody (CAB10615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s. |
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