Name: Anti-CYP4V2 Antibody (CAB6573)
Brand: Metabolism Antibodies 2
SKU: CAB6573
Availability: OutOfStock

Antibody Name:	Anti-CYP4V2 Antibody
Antibody SKU:	CAB6573
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 246-525 of human CYP4V2 (NP_997235.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	THP-1, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 246-525 of human CYP4V2 (NP_997235.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LMFK EGWE HKKS LQIL HTFT NSVI AERA NEMN ANED CRGD GRGS APSK NKRR AFLD LLLS VTDD EGNR LSHE DIRE EVDT FMFE GHDT TAAA INWS LYLL GSNP EVQK KVDH ELDD VFGK SDRP ATVE DLKK LRYL ECVI KETL RLFP SVPL FARS VSED CEVA GYRV LKGT EAVI IPYA LHRD PRYF PNPE EFQP ERFF PENA QGRH PYAY VPFS AGPR NCIG QKFA VMEE KTIL SCIL RHFW IESN QKRE ELGL EGQL ILRP SNGI WIKL KRRN ADER
Gene ID:	285440
Uniprot:	Q6ZWL3
Cellular Location:	Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:	58kDa/60kDa
Observed MW:	54kDa

Synonyms:	CYP4V2, BCD, CYP4AH1
Background:	This gene encodes a member of the cytochrome P450 hemethiolate protein superfamily which are involved in oxidizing various substrates in the metabolic pathway. It is implicated in the metabolism of fatty acid precursors into n-3 polyunsaturated fatty acids. Mutations in this gene result in Bietti crystalline corneoretinal dystrophy.

UniProt Protein Function:	CYP4V2: Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12). May have a role in fatty acid and steroid metabolism in the eye. Defects in CYP4V2 are a cause of Bietti crystalline corneoretinal dystrophy (BCD). BCD is an autosomal recessive retinal dystrophy characterized by multiple glistening intraretinal crystals scattered over the fundus, a characteristic degeneration of the retina, and sclerosis of the choroidal vessels, ultimately resulting in progressive night blindness and constriction of the visual field. Most cases have similar crystals at the corneoscleral limbus. Clinically, BCD is progressive. Patients develop decreased vision, nyctalopia, and paracentral scotomata between the 2nd and 4th decade of life. Later, patients develop peripheral visual field loss and marked visual impairment, usually progressing to legal blindness by the 5th or 6th decade of life. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.14.13.199; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q35.2 Cellular Component: endoplasmic reticulum membrane Molecular Function:monooxygenase activity Biological Process: fatty acid omega-oxidation; retinoid metabolic process; sterol metabolic process Disease: Bietti Crystalline Corneoretinal Dystrophy
NCBI Summary:	This gene encodes a member of the cytochrome P450 hemethiolate protein superfamily which are involved in oxidizing various substrates in the metabolic pathway. It is implicated in the metabolism of fatty acid precursors into n-3 polyunsaturated fatty acids. Mutations in this gene result in Bietti crystalline corneoretinal dystrophy. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6ZWL3
NCBI GenInfo Identifier:	296434466
NCBI Gene ID:	285440
NCBI Accession:	Q6ZWL3.2
UniProt Secondary Accession:	Q6ZWL3,Q6ZTM4, B7U6W2,
UniProt Related Accession:	Q6ZWL3
Molecular Weight:	58,166 Da
NCBI Full Name:	Cytochrome P450 4V2
NCBI Synonym Full Names:	cytochrome P450 family 4 subfamily V member 2
NCBI Official Symbol:	CYP4V2
NCBI Official Synonym Symbols:	BCD; CYP4AH1
NCBI Protein Information:	cytochrome P450 4V2
UniProt Protein Name:	Cytochrome P450 4V2
UniProt Synonym Protein Names:	Docosahexaenoic acid omega-hydroxylase CYP4V2
Protein Family:	Cytochrome
UniProt Gene Name:	CYP4V2
UniProt Entry Name:	CP4V2_HUMAN

Western blot analysis of extracts of various cell lines, using CYP4V2 Antibody (CAB6573) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

CYP4V2 Antibody (PACO03945)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)