Metabolism Antibodies 2

Anti-CYP4F12 Antibody (CAB7993)

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SKU:
CAB7993

Description

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Antibody Name:CYP4F12 Rabbit Polyclonal Antibody
Antibody SKU:CAB7993
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 265-524 of human CYP4F12 (NP_076433.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse placenta, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 265-524 of human CYP4F12 (NP_076433.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DFTD AVIR ERRR TLPT QGID DFFK DKAK SKTL DFID VLLL SKDE DGKA LSDE DIRA EADT FMFG GHDT TASG LSWV LYNL ARHP EYQE RCRQ EVQE LLKD RDPK EIEW DDLA QLPF LTMC VKES LRLH PPAP FISR CCTQ DIVL PDGR VIPK GITC LIDI IGVH HNPT VWPD PEVY DPFR FDPE NSKG RSPL AFIP FSAG PRNC IGQA FAMA EMKV VLAL MLLH FRFL PDHT EPRR KLEL IMRA EGGL WLRV EPLN VSLQ
Gene ID:66002
Uniprot:Q9HCS2
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Multi-pass membrane protein
Calculated MW:11kDa/60kDa
Observed MW:60kDa
Synonyms:CYP4F12, CYPIVF12, F22329_1
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CYP4F12: Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:Membrane protein, integral; EC 1.14.13.30; Membrane protein, multi-pass; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: apical plasma membrane; cytoplasm; endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function:alkane 1-monooxygenase activity; arachidonic acid epoxygenase activity; leukotriene-B4 20-monooxygenase activity; oxygen binding

Biological Process: arachidonic acid metabolic process; drug metabolic process; epoxygenase P450 pathway; long-chain fatty acid metabolic process; pressure natriuresis; renal water homeostasis; sodium ion homeostasis; very-long-chain fatty acid metabolic process; vitamin E metabolic process

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum. When expressed in yeast the enzyme is capable of oxdizing arachidonic acid. It can also catalyze the epoxidation of 22:6n-3 and 22:5n-3 polyunsaturated long-chain fatty acids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Code:Q9HCS2
NCBI GenInfo Identifier:313104094
NCBI Gene ID:66002
NCBI Accession:Q9HCS2.2
UniProt Secondary Accession:Q9HCS2,O60389, Q5JPJ7, Q9HCS1, E7ET51,
UniProt Related Accession:Q9HCS2
Molecular Weight:11,572 Da
NCBI Full Name:Cytochrome P450 4F12
NCBI Synonym Full Names:cytochrome P450 family 4 subfamily F member 12
NCBI Official Symbol:CYP4F12  
NCBI Official Synonym Symbols:CYPIVF12; F22329_1  
NCBI Protein Information:cytochrome P450 4F12
UniProt Protein Name:Cytochrome P450 4F12
UniProt Synonym Protein Names:CYPIVF12
Protein Family:Cytochrome
UniProt Gene Name:CYP4F12  
UniProt Entry Name:CP4FC_HUMAN
Anti-CYP4F12 Polyclonal Antibody (CAB7993)Western blot analysis of extracts of various cell lines, using CYP4F12 antibody (CAB7993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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Additional Information

Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
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