Antibody Name:	Anti-CYP46A1 Antibody
Antibody SKU:	CAB8573
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, U-251MG, MCF7, Mouse liver, Mouse brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HRAR SRYE HIPG PPRP SFLL GHLP CFWK KDEV GGRV LQDV FLDW AKKY GPVV RVNV FHKT SVIV TSPE SVKK FLMS TKYN KDSK MYRA LQTV FGER LFGQ GLVS ECNY ERWH KQRR VIDL AFSR SSLV SLME TFNE KAEQ LVEI LEAK ADGQ TPVS MQDM LTYT AMDI LAKA AFGM ETSM LLGA QKPL SQAV KLML EGIT ASRN TLAK FLPG KRKQ LREV RESI RFLR QVGR DWVQ RRRE ALKR GEEV PADI LTQI L
Gene ID:	10858
Uniprot:	Q9Y6A2
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein
Calculated MW:	38kDa/45kDa/56kDa
Observed MW:	57kDa

Synonyms:

CYP46A1, CP46, CYP46

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.

UniProt Protein Function:	CSTB: This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1). EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. Belongs to the cystatin family.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: extracellular space; cytoplasm; nucleolus Molecular Function:protease binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity Biological Process: negative regulation of proteolysis; regulation of apoptosis; adult locomotory behavior; negative regulation of peptidase activity Disease: Myoclonic Epilepsy Of Unverricht And Lundborg
UniProt Code:	Q9Y6A2
NCBI GenInfo Identifier:	5729796
NCBI Gene ID:	10858
NCBI Accession:	NP_006659
UniProt Secondary Accession:	Q9Y6A2,Q8N2B0, B4DHP8, E7EQG9,
UniProt Related Accession:	Q9Y6A2
Molecular Weight:	38,315 Da
NCBI Full Name:	cholesterol 24-hydroxylase
NCBI Synonym Full Names:	cytochrome P450, family 46, subfamily A, polypeptide 1
NCBI Official Symbol:	CYP46A1
NCBI Official Synonym Symbols:	CP46; CYP46
NCBI Protein Information:	cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
UniProt Protein Name:	Cystatin-B
UniProt Synonym Protein Names:	CPI-B; Liver thiol proteinase inhibitor; Stefin-B
Protein Family:	Cystatin
UniProt Gene Name:	CSTB
UniProt Entry Name:	CYTB_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP46A1 antibody (CAB8573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human prostate using CYP46A1 antibody (CAB8573) at dilution of 1:100 (40x lens).