Antibody Name:	Anti-CYP3A7 Antibody
Antibody SKU:	CAB10026
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A7 (NP_000756.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HT-29, Mouse liver, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A7 (NP_000756.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVIS FLTK SVKQ IKEG RLKE TQKH RVDF LQLM IDSQ NSKD SETH KALS DLEL MAQS IIFI FAGY ETTS SVLS FIIY ELAT HPDV QQKV QKEI DTVL PNKA PPTY DTVL QLEY LDMV VNET LRLF PVAM RLER VCKK DVEI NGMF IPKG VVVM IPSY VLHH DPKY WTEP EKFL PERF SKKN KDNI DPYI YTPF GSGP RNCI GMRF ALVN MKLA LVRV LQNF SFKP CKET QIPL KLRF GGLL LTEK PIVL KAES RDET VSGA
Gene ID:	1551
Uniprot:	P24462
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:	57kDa/61kDa
Observed MW:	75kDa

Synonyms:

CYP3A7, CP37, CYPIIIA7, P-450(HFL33), P-450111A7, P450-HFLA

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540.

UniProt Protein Function:	CYP3A7: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1 Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: endoplasmic reticulum membrane Molecular Function:iron ion binding; heme binding; oxygen binding; monooxygenase activity Biological Process: xenobiotic metabolic process
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540. [provided by RefSeq, Jan 2015]
UniProt Code:	P24462
NCBI GenInfo Identifier:	608788370
NCBI Gene ID:	1551
NCBI Accession:	NP_000756.3
UniProt Secondary Accession:	P24462,Q9H241, A4D288,
UniProt Related Accession:	P24462
Molecular Weight:	61,547 Da
NCBI Full Name:	cytochrome P450 3A7
NCBI Synonym Full Names:	cytochrome P450, family 3, subfamily A, polypeptide 7
NCBI Official Symbol:	CYP3A7
NCBI Official Synonym Symbols:	CP37; CYPIIIA7; P450-HFLA; P-450111A7; P-450(HFL33)
NCBI Protein Information:	cytochrome P450 3A7
UniProt Protein Name:	Cytochrome P450 3A7
UniProt Synonym Protein Names:	CYPIIIA7; Cytochrome P450-HFLA
Protein Family:	Cytochrome
UniProt Gene Name:	CYP3A7
UniProt Entry Name:	CP3A7_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP3A7 antibody (CAB10026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using CYP3A7 antibody (CAB10026) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.