Metabolism Antibodies 2

Anti-CYP3A5 Antibody (CAB7663)

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SKU:
CAB7663
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-CYP3A5 Antibody
Antibody SKU:CAB7663
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 233-502 of human CYP3A5 (NP_000768.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 233-502 of human CYP3A5 (NP_000768.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FEAL NVSL FPKD TINF LSKS VNRM KKSR LNDK QKHR LDFL QLMI DSQN SKET ESHK ALSD LELA AQSI IFIF AGYE TTSS VLSF TLYE LATH PDVQ QKLQ KEID AVLP NKAP PTYD AVVQ MEYL DMVV NETL RLFP VAIR LERT CKKD VEIN GVFI PKGS MVVI PTYA LHHD PKYW TEPE EFRP ERFS KKKD SIDP YIYT PFGT GPRN CIGM RFAL MNMK LALI RVLQ NFSF KPCK ETQI PLKL DTQG LLQP EKPI VLKV DSRD GTLS GE
Gene ID:1577
Uniprot:P20815
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:15kDa/57kDa
Observed MW:57kDa
Synonyms:CYP3A5, CP35, CYPIIIA5, P450PCN3, PCN3
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CYP3A5: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.14.14.1; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function:monooxygenase activity; oxidoreductase activity; oxygen binding

Biological Process: alkaloid catabolic process; drug catabolic process; steroid metabolic process; xenobiotic metabolic process

Disease: Hypertension, Essential

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Code:P20815
NCBI GenInfo Identifier:117157
NCBI Gene ID:1577
NCBI Accession:P20815.1
UniProt Secondary Accession:P20815,Q53WY8, Q75MV0, Q9HB56, A4D289, B7Z5I7,
UniProt Related Accession:P20815
Molecular Weight:15,846 Da
NCBI Full Name:Cytochrome P450 3A5
NCBI Synonym Full Names:cytochrome P450 family 3 subfamily A member 5
NCBI Official Symbol:CYP3A5  
NCBI Official Synonym Symbols:CP35; PCN3; CYPIIIA5; P450PCN3  
NCBI Protein Information:cytochrome P450 3A5
UniProt Protein Name:Cytochrome P450 3A5
UniProt Synonym Protein Names:CYPIIIA5; Cytochrome P450 HLp2; Cytochrome P450-PCN3
UniProt Gene Name:CYP3A5  
UniProt Entry Name:CP3A5_HUMAN
Anti-CYP3A5 Antibody (CAB7663)Western blot analysis of extracts of Rat thymus, using CYP3A5 antibody (CAB7663) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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