KO Validated Antibodies 1

Anti-CYP3A4 Antibody (CAB13484)[KO Validated]

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SKU:
CAB13484
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-CYP3A4 Antibody [KO Validated]
Antibody SKU:CAB13484
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Rat liver, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EVTN FLRK SVKR MKES RLED TQKH RVDF LQLM IDSQ NSKE TESH KALS DLEL VAQS IIFI FAGY ETTS SVLS FIMY ELAT HPDV QQKL QEEI DAVL PNKA PPTY DTVL QMEY LDMV VNET LRLF PIAM RLER VCKK DVEI NGMF IPKG VVVM IPSY ALHR DPKY WTEP EKFL PERF SKKN KDNI DPYI YTPF GSGP RNCI GMRF ALMN MKLA LIRV LQNF SFKP CKET QIPL KLSL GGLL QPEK PVVL KVES RDGT VSGA
Gene ID:1576
Uniprot:P08684
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein
Calculated MW:57kDa
Observed MW:50kDa
Synonyms:CYP3A4, CP33, CP34, CYP3A, CYP3A3, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450C3, P450PCN1
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cell surface; Oxidoreductase; Membrane protein, integral; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - other enzymes; Lipid Metabolism - linoleic acid; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm; integral to membrane

Molecular Function:quinine 3-monooxygenase activity; albendazole monooxygenase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxidoreductase activity; oxygen binding; vitamin D3 25-hydroxylase activity; testosterone 6-beta-hydroxylase activity; steroid binding; enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; monooxygenase activity

Biological Process: steroid metabolic process; drug catabolic process; xenobiotic metabolic process; androgen metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; alkaloid catabolic process; heterocycle metabolic process; lipid metabolic process; drug metabolic process; vitamin D metabolic process; steroid catabolic process

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]
UniProt Code:P08684
NCBI GenInfo Identifier:116241312
NCBI Gene ID:1576
NCBI Accession:P08684.4
UniProt Secondary Accession:P08684,P05184, Q16757, Q9UK50,
UniProt Related Accession:P08684
Molecular Weight:503
NCBI Full Name:Cytochrome P450 3A4
NCBI Synonym Full Names:cytochrome P450, family 3, subfamily A, polypeptide 4
NCBI Official Symbol:CYP3A4  
NCBI Official Synonym Symbols:HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1  
NCBI Protein Information:cytochrome P450 3A4; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; 1,8-cineole 2-exo-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
UniProt Protein Name:Cytochrome P450 3A4
UniProt Synonym Protein Names:1,8-cineole 2-exo-monooxygenase (EC:1.14.13.157); Albendazole monooxygenase (EC:1.14.13.32); Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase (EC:1.14.13.67); Taurochenodeoxycholate 6-alpha-hydroxylase (EC:1.14.13.97)
Protein Family:Cytochrome
UniProt Gene Name:CYP3A4  
UniProt Entry Name:CP3A4_HUMAN
Anti-CYP3A4 Antibody [KO Validated] (CAB13484)Western blot analysis of extracts of various cell lines, using CYP3A4 antibody (CAB13484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-CYP3A4 Antibody [KO Validated] (CAB13484)Western blot analysis of extracts from normal (control) and CYP3A4 Rabbit pAb knockout (KO) 293T cells, using CYP3A4 Rabbit pAb antibody (CAB13484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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