Antibody Name:	Anti-CYP2F1 Antibody
Antibody SKU:	CAB7550
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-290 of human CYP2F1 (NP_000765.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, BT-474, Mouse lung, Mouse liver, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-290 of human CYP2F1 (NP_000765.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DYPA FFNF TKGN GIAF SSGD RWKV LRQF SIQI LRNF GMGK RSIE ERIL EEGS FLLA ELRK TEGE PFDP TFVL SRSV SNII CSVL FGSR FDYD DERL LTII RLIN DNFQ IMSS PWGE LYDI FPSL LDWV PGPH QRIF QNFK CLRD LIAH SVHD HQAS LDPR SPRD FIQC FLTK MAEE KEDP LSHF HMDT LLM
Gene ID:	1572
Uniprot:	P24903
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:	36kDa/55kDa
Observed MW:	46-54kDa

Synonyms:

CYP2F1, C2F1, CYP2F, CYPIIF1

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.

UniProt Protein Function:	CYP2F2: May be involved in the metabolism of various pneumotoxicants including naphthalene. Is able to dealkylate ethoxycoumarin, propoxycoumarin, and pentoxyresorufin but possesses no activity toward ethoxyresorufin and only trace dearylation activity toward benzyloxyresorufin. Bioactivates 3- methylindole (3MI) by dehydrogenation to the putative electrophile 3-methylene-indolenine. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Oxidoreductase Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm Molecular Function:iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding; monooxygenase activity Biological Process: response to toxin; xenobiotic metabolic process; exogenous drug catabolic process; trichloroethylene metabolic process; epoxygenase P450 pathway; naphthalene metabolic process
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
UniProt Code:	P24903
NCBI GenInfo Identifier:	259016202
NCBI Gene ID:	1572
NCBI Accession:	P24903.2
UniProt Related Accession:	P24903
Molecular Weight:
NCBI Full Name:	Cytochrome P450 2F1
NCBI Synonym Full Names:	cytochrome P450 family 2 subfamily F member 1
NCBI Official Symbol:	CYP2F1
NCBI Official Synonym Symbols:	C2F1; CYP2F; CYPIIF1
NCBI Protein Information:	cytochrome P450 2F1
UniProt Protein Name:	Cytochrome P450 2F1
UniProt Synonym Protein Names:	CYPIIF1
UniProt Gene Name:	CYP2F1
UniProt Entry Name:	CP2F1_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP2F1 antibody (CAB7550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using CYP2F1 antibody (CAB7550) at dilution of 1:200 (40x lens).