Antibody Name:	Anti-CYP2C9 Antibody
Antibody SKU:	CAB6219
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 211-490 of human CYP2C9 (NP_000762.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-549, MCF7, U-937, HepG2, 22Rv1, Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 211-490 of human CYP2C9 (NP_000762.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PWIQ ICNN FSPI IDYF PGTH NKLL KNVA FMKS YILE KVKE HQES MDMN NPQD FIDC FLMK MEKE KHNQ PSEF TIES LENT AVDL FGAG TETT STTL RYAL LLLL KHPE VTAK VQEE IERV IGRN RSPC MQDR SHMP YTDA VVHE VQRY IDLL PTSL PHAV TCDI KFRN YLIP KGTT ILIS LTSV LHDN KEFP NPEM FDPH HFLD EGGN FKKS KYFM PFSA GKRI CVGE ALAG MELF LFLT SILQ NFNL KSLV DPKN LDTT PVVN GFAS VPPF YQLC FIPV
Gene ID:	1559
Uniprot:	P11712
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Calculated MW:	18kDa/55kDa
Observed MW:	63kDa

Synonyms:

CYP2C9, CPC9, CYP2C, CYP2C10, CYPIIC9, P450IIC9

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24.

UniProt Protein Function:	CYP2C9: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S- warfarin, diclofenac, phenytoin, tolbutamide and losartan. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; EC 1.14.14.1; Secondary Metabolites Metabolism - limonene and pinene degradation; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450 Chromosomal Location of Human Ortholog: 10q24 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm Molecular Function:(S)-limonene 6-monooxygenase activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; (S)-limonene 7-monooxygenase activity; oxidoreductase activity; drug binding; steroid hydroxylase activity; oxygen binding; monooxygenase activity Biological Process: steroid metabolic process; amide metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; drug metabolic process; monocarboxylic acid metabolic process; urea metabolic process Disease: Coumarin Resistance
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]
UniProt Code:	P11712
NCBI GenInfo Identifier:	6686268
NCBI Gene ID:	1559
NCBI Accession:	P11712.3
UniProt Secondary Accession:	P11712,P11713, Q16756, Q16872, Q5VX92, Q6IRV8, Q8WW80
UniProt Related Accession:	P11712
Molecular Weight:	Calculated MW: 18kDa/55kDaObserved MW: 63kDa
NCBI Full Name:	Cytochrome P450 2C9
NCBI Synonym Full Names:	cytochrome P450, family 2, subfamily C, polypeptide 9
NCBI Official Symbol:	CYP2C9
NCBI Official Synonym Symbols:	CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
NCBI Protein Information:	cytochrome P450 2C9; cytochrome P-450MP; cytochrome P450 PB-1; microsomal monooxygenase; xenobiotic monooxygenase; flavoprotein-linked monooxygenase; cytochrome P-450 S-mephenytoin 4-hydroxylase
UniProt Protein Name:	Cytochrome P450 2C9
UniProt Synonym Protein Names:	(R)-limonene 6-monooxygenase (EC:1.14.13.80); (S)-limonene 6-monooxygenase (EC:1.14.13.48); (S)-limonene 7-monooxygenase (EC:1.14.13.49); CYPIIC9; Cytochrome P-450MP; Cytochrome P450 MP-4; Cytochrome P450 MP-8; Cytochrome P450 PB-1; S-mephenytoin 4-hydroxylase
Protein Family:	Cytochrome
UniProt Gene Name:	CYP2C9
UniProt Entry Name:	CP2C9_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP2C9 antibody (CAB6219) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse lung using CYP2C9 Antibody (CAB6219) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using CYP2C9 antibody (CAB6219). Blue: DAPI for nuclear staining.