Cell Biology Antibodies 5
Anti-CYP2B6 Antibody (CAB1463)
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- SKU:
- CAB1463
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CYP2B6 Antibody |
| Antibody SKU: | CAB1463 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_000758.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, HT-29, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-340 of human CYP2B6 (NP_000758.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SKGA LMDP TFLF QSIT ANII CSIV FGKR FHYQ DQEF LKML NLFY QTFS LISS VFGQ LFEL FSGF LKYF PGAH RQVY KNLQ EINA YIGH SVEK HRET LDPS APKD LIDT YLLH MEKE KSNA HSEF SHQN LNLN TLSL FFAG TETT STTL RYGF LLML KYPH VAER VYRE IEQV IGPH RPPE L |
| Gene ID: | 1555 |
| Uniprot: | P20813 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein |
| Calculated MW: | 33kDa/56kDa |
| Observed MW: | 66kDa |
| Synonyms: | CYP2B6, CPB6, CYP2B, CYP2B7, CYP2B7P, CYPIIB6, EFVM, IIB1, P450 |
| Background: | This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. |
| UniProt Protein Function: | CYP2B6: cytochrome P450, subfamily IIB. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Phosphorylation is accompanied by a decrease in enzyme activity. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Lipid Metabolism - arachidonic acid Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: endoplasmic reticulum membrane Molecular Function:arachidonic acid epoxygenase activity; heme binding; monooxygenase activity; oxygen binding; steroid hydroxylase activity Biological Process: drug metabolic process; epoxygenase P450 pathway; exogenous drug catabolic process; ketone metabolic process; steroid metabolic process; xenobiotic metabolic process Disease: Efavirenz, Poor Metabolism Of |
| NCBI Summary: | This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P20813 |
| NCBI GenInfo Identifier: | 117205 |
| NCBI Gene ID: | 1555 |
| NCBI Accession: | P20813.1 |
| UniProt Secondary Accession: | P20813,Q2V565, Q9UK46, B4DWP3, |
| UniProt Related Accession: | P20813 |
| Molecular Weight: | 33,385 Da |
| NCBI Full Name: | Cytochrome P450 2B6 |
| NCBI Synonym Full Names: | cytochrome P450 family 2 subfamily B member 6 |
| NCBI Official Symbol: | CYP2B6 |
| NCBI Official Synonym Symbols: | CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6 |
| NCBI Protein Information: | cytochrome P450 2B6 |
| UniProt Protein Name: | Cytochrome P450 2B6 |
| UniProt Synonym Protein Names: | 1,4-cineole 2-exo-monooxygenase; CYPIIB6; Cytochrome P450 IIB1 |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYP2B6 |
| UniProt Entry Name: | CP2B6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP2B6 antibody (CAB1463) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using CYP2B6 antibody (CAB1463) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using CYP2B6 antibody (CAB1463) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using CYP2B6 antibody (CAB1463) at dilution of 1:100 (40x lens). |
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