Cell Biology Antibodies 7

Anti-CYP24A1 Antibody (CAB1805)

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SKU:
CAB1805
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CYP24A1 Antibody
Antibody SKU:CAB1805
Antibody Size:20uL, 50uL, 100uL
Application:IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 36-448 of human CYP24A1 (NP_001122387.1).
Application:IHC IF
Recommended Dilution:IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 36-448 of human CYP24A1 (NP_001122387.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PQPR EVPV CPLT AGGE TQNA AALP GPTS WPLL GSLL QILW KGGL KKQH DTLV EYHK KYGK IFRM KLGS FESV HLGS PCLL EALY RTES AYPQ RLEI KPWK AYRD YRKE GYGL LILE GEDW QRVR SAFQ KKLM KPGE VMKL DNKI NEVL ADFM GRID ELCD ERGH VEDL YSEL NKWS FESI CLVL YEKR FGLL QKNA GDEA VNFI MAIK TMMS TFGR MMVT PVEL HKSL NTKV WQDH TLAW DTIF KSVK ACID NRLE KYSQ QPSA DFLC DIYH QNRL SKKE LYAA VTEL QLAA VETT ANSL MWIL YNLS RNPQ VQQK LLKE IQSV LPEN QVPR AEDL RNMP YLKA CLKE SMRL TPSV PFTT RTLD KATV LGEY ALPK GIVR KYDI QATD NEPV EMLH SGTL VPSR ELPI AFCQ R
Gene ID:1591
Uniprot:Q07973
Cellular Location:Mitochondrion
Calculated MW:43kDa/51kDa/58kDa
Observed MW:Refer to figures
Synonyms:CYP24A1, CP24, CYP24, HCAI, HCINF1, P450-CC24
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1, 25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.14.13.126; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: mitochondrial inner membrane

Molecular Function:iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: osteoblast differentiation; steroid metabolic process; response to vitamin D; vitamin metabolic process; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process

Disease: Hypercalcemia, Infantile

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q07973
NCBI GenInfo Identifier:19862747
NCBI Gene ID:1591
NCBI Accession:Q07973.2
UniProt Secondary Accession:Q07973,Q15807, Q32ML3, Q5I2W7,
UniProt Related Accession:Q07973
Molecular Weight:59kDa
NCBI Full Name:1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
NCBI Synonym Full Names:cytochrome P450, family 24, subfamily A, polypeptide 1
NCBI Official Symbol:CYP24A1  
NCBI Official Synonym Symbols:CP24; HCAI; CYP24; P450-CC24  
NCBI Protein Information:1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
UniProt Protein Name:1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Synonym Protein Names:Cytochrome P450 24A1; Cytochrome P450-CC24
Protein Family:1,25-dihydroxyvitamin D(3) 24-hydroxylase
UniProt Gene Name:CYP24A1  
UniProt Entry Name:CP24A_HUMAN
Anti-CYP24A1 Antibody (CAB1805)Immunohistochemistry of paraffin-embedded rat pancreas using CYP24A1 antibody (CAB1805) at dilution of 1:200 (40x lens).
Anti-CYP24A1 Antibody (CAB1805)Immunohistochemistry of paraffin-embedded mouse kidney using CYP24A1 antibody (CAB1805) at dilution of 1:200 (40x lens).
Anti-CYP24A1 Antibody (CAB1805)Immunohistochemistry of paraffin-embedded human liver cancer using CYP24A1 antibody (CAB1805) at dilution of 1:200 (40x lens).
Anti-CYP24A1 Antibody (CAB1805)Immunofluorescence analysis of NIH-3T3 cells using CYP24A1 Polyclonal Antibody (CAB1805) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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