Cell Biology Antibodies 10
Anti-CYP20A1 Antibody (CAB6476)
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- SKU:
- CAB6476
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CYP20A1 Antibody |
| Antibody SKU: | CAB6476 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 183-462 of human CYP20A1 (NP_803882.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse lung, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 183-462 of human CYP20A1 (NP_803882.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | STFE DDQE VIRF QKNH GTVW SEIG KGFL DGSL DKNM TRKK QYED ALMQ LESV LRNI IKER KGRN FSQH IFID SLVQ GNLN DQQI LEDS MIFS LASC IITA KLCT WAIC FLTT SEEV QKKL YEEI NQVF GNGP VTPE KIEQ LRYC QHVL CETV RTAK LTPV SAQL QDIE GKID RFII PRET LVLY ALGV VLQD PNTW PSPH KFDP DRFD DELV MKTF SSLG FSGT QECP ELRF AYMV TTVL LSVL VKRL HLLS VEGQ VIET KYEL VTSS REEA WITV SKRY |
| Gene ID: | 57404 |
| Uniprot: | Q6UW02 |
| Cellular Location: | Membrane, Single-pass membrane protein |
| Calculated MW: | 49kDa/52kDa |
| Observed MW: | 46kDa |
| Synonyms: | CYP20A1, CYP-M |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D, E)T, suggesting that its substrate may carry its own oxygen. |
| UniProt Protein Function: | CYP20A1: a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:EC 1.14.-.-; Oxidoreductase; Membrane protein, integral Chromosomal Location of Human Ortholog: 2q33.2 Cellular Component: membrane |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6UW02 |
| NCBI GenInfo Identifier: | 74749375 |
| NCBI Gene ID: | 57404 |
| NCBI Accession: | Q6UW02.1 |
| UniProt Secondary Accession: | Q6UW02,Q4ZG61, Q8N4Q8, Q8WWA9, Q9HC04, |
| UniProt Related Accession: | Q6UW02 |
| Molecular Weight: | 49,428 Da |
| NCBI Full Name: | Cytochrome P450 20A1 |
| NCBI Synonym Full Names: | cytochrome P450 family 20 subfamily A member 1 |
| NCBI Official Symbol: | CYP20A1 |
| NCBI Official Synonym Symbols: | CYP-M |
| NCBI Protein Information: | cytochrome P450 20A1 |
| UniProt Protein Name: | Cytochrome P450 20A1 |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYP20A1 |
| UniProt Entry Name: | CP20A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP20A1 antibody (CAB6476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using CYP20A1 antibody (CAB6476) at dilution of 1:200 (40x lens). |
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