Metabolism Antibodies 1
Anti-CYP1B1 Antibody (CAB1377)
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- SKU:
- CAB1377
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CYP1B1 Antibody |
| Antibody SKU: | CAB1377 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CYP1B1 (NP_000095.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, MCF7, HL-60, HT-29, Mouse kidney, Mouse heart, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CYP1B1 (NP_000095.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGTS LSPN DPWP LNPL SIQQ TTLL LLLS VLAT VHVG QRLL RQRR RQLR SAPP GPFA WPLI GNAA AVGQ AAHL SFAR LARR YGDV FQIR LGSC PIVV LNGE RAIH QALV QQGS AFAD RPAF ASFR VVSG GRSM AFGH YSEH WKVQ RRAA HSMM RNFF TRQP RSRQ VLEG HVLS EARE LVAL LVRG SADG AFLD PRPL TVVA VANV MSAV CFGC RYSH DDPE FREL LSHN EEFG RTVG AGSL VDVM PWLQ YFPN PVRT VFRE |
| Gene ID: | 1545 |
| Uniprot: | Q16678 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion, Peripheral membrane protein |
| Calculated MW: | 60kDa |
| Observed MW: | 60kDa |
| Synonyms: | CYP1B1, ASGD6, CP1B, CYPIB1, GLC3A, P4501B1 |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. |
| UniProt Protein Function: | CYP1B1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A). GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG). POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes. Defects in CYP1B1 are a cause of Peters anomaly (PAN). Peters anomaly is a congenital defect of the anterior chamber of the eye. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Amino Acid Metabolism - tryptophan Chromosomal Location of Human Ortholog: 2p22.2 Cellular Component: endoplasmic reticulum membrane; mitochondrion Molecular Function:iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; monooxygenase activity Biological Process: steroid metabolic process; estrogen metabolic process; collagen fibril organization; retinal metabolic process; positive regulation of apoptosis; response to toxin; positive regulation of JAK-STAT cascade; negative regulation of cell proliferation; visual perception; retinol metabolic process; arachidonic acid metabolic process; angiogenesis; nitric oxide biosynthetic process; cell adhesion; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; epoxygenase P450 pathway; positive regulation of angiogenesis; inhibition of NF-kappaB transcription factor; xenobiotic metabolic process; toxin metabolic process; endothelial cell migration; blood vessel morphogenesis; aromatic compound metabolic process; membrane lipid catabolic process; induction of apoptosis by oxidative stress; sterol metabolic process Disease: Peters Anomaly; Glaucoma 3, Primary Congenital, A; Glaucoma 3, Primary Infantile, B |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q16678 |
| NCBI GenInfo Identifier: | 48429256 |
| NCBI Gene ID: | 1545 |
| NCBI Accession: | Q16678.2 |
| UniProt Secondary Accession: | Q16678,Q5TZW8, Q93089, Q9H316, |
| UniProt Related Accession: | Q16678 |
| Molecular Weight: | |
| NCBI Full Name: | Cytochrome P450 1B1 |
| NCBI Synonym Full Names: | cytochrome P450, family 1, subfamily B, polypeptide 1 |
| NCBI Official Symbol: | CYP1B1Â Â |
| NCBI Official Synonym Symbols: | CP1B; GLC3A; CYPIB1; P4501B1Â Â |
| NCBI Protein Information: | cytochrome P450 1B1; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile) |
| UniProt Protein Name: | Cytochrome P450 1B1 |
| UniProt Synonym Protein Names: | CYPIB1 |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYP1B1Â Â |
| UniProt Entry Name: | CP1B1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYP1B1 antibody (CAB1377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using CYP1B1 antibody (CAB1377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using CYP1B1 antibody (CAB1377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of PC-12 cells using CYP1B1 antibody (CAB1377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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