Metabolism Antibodies 1
Anti-CYP1A2 Antibody (CAB0062)
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- SKU:
- CAB0062
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CYP1A2 Antibody |
| Antibody SKU: | CAB0062 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-305 of human CYP1A2 (NP_000752.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-305 of human CYP1A2 (NP_000752.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FGQH FPES SDEM LSLV KNTH EFVE TASS GNPL DFFP ILRY LPNP ALQR FKAF NQRF LWFL QKTV QEHY QDFD KNSV RDIT GALF KHSK KGPR ASGN LIPQ E |
| Gene ID: | 1544 |
| Uniprot: | P05177 |
| Cellular Location: | Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein |
| Calculated MW: | 58kDa |
| Observed MW: | 54kDa |
| Synonyms: | CYP1A2, CP12, P3-450, P450(PA), CYPIA2 |
| Background: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. |
| UniProt Protein Function: | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05177 |
| NCBI GenInfo Identifier: | 1476413392 |
| NCBI Gene ID: | 1544 |
| NCBI Accession: | P05177.4 |
| UniProt Secondary Accession: | P05177,Q16754, Q6NWU3, Q6NWU5, Q9BXX7, Q9UK49, |
| UniProt Related Accession: | P05177 |
| Molecular Weight: | 57 KD |
| NCBI Full Name: | Cytochrome P450 1A2 |
| NCBI Synonym Full Names: | cytochrome P450 family 1 subfamily A member 2 |
| NCBI Official Symbol: | CYP1A2 |
| NCBI Official Synonym Symbols: | CP12; CYPIA2; P3-450; P450(PA) |
| NCBI Protein Information: | cytochrome P450 1A2 |
| UniProt Protein Name: | Cytochrome P450 1A2 |
| UniProt Synonym Protein Names: | CYPIA2; Cholesterol 25-hydroxylase |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYP1A2 |
 | Western blot analysis of extracts of various cell lines, using CYP1A2 antibody (CAB0062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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