Cell Cycle Antibodies 2
Anti-Cyclin E2 Antibody (CAB7032)
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- SKU:
- CAB7032
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-Cyclin E2 Antibody |
| Antibody SKU: | CAB7032 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 255-404 of human Cyclin E2 (NP_477097.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 255-404 of human Cyclin E2 (NP_477097.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ALKD APKV LLPQ YSQE TFIQ IAQL LDLC ILAI DSLE FQYR ILTA AALC HFTS IEVV KKAS GLEW DSIS ECVD WMVP FVNV VKST SPVK LKTF KKIP MEDR HNIQ THTN YLAM LEEV NYIN TFRK GGQL SPVC NGGI MTPP KSTE KPPG KH |
| Gene ID: | 9134 |
| Uniprot: | O96020 |
| Cellular Location: | Nucleus |
| Calculated MW: | 41kDa/46kDa |
| Observed MW: | 47kDa |
| Synonyms: | CCNE2, CYCE2, cyclin E2 |
| Background: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. |
| UniProt Protein Function: | CCNE2: Essential for the control of the cell cycle at the late G1 and early S phase. Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively. According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor- derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Belongs to the cyclin family. Cyclin E subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Activator Chromosomal Location of Human Ortholog: 8q22.1 Cellular Component: nucleoplasm; cytosol Molecular Function:protein binding; cyclin-dependent protein kinase regulator activity Biological Process: DNA replication initiation; cell division; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; cell cycle checkpoint; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O96020 |
| NCBI GenInfo Identifier: | 5921733 |
| NCBI Gene ID: | 9134 |
| NCBI Accession: | O96020.1 |
| UniProt Secondary Accession: | O96020,O95439, |
| UniProt Related Accession: | O96020 |
| Molecular Weight: | 404 |
| NCBI Full Name: | G1/S-specific cyclin-E2 |
| NCBI Synonym Full Names: | cyclin E2 |
| NCBI Official Symbol: | CCNE2 |
| NCBI Official Synonym Symbols: | CYCE2 |
| NCBI Protein Information: | G1/S-specific cyclin-E2 |
| UniProt Protein Name: | G1/S-specific cyclin-E2 |
| Protein Family: | G1/S-specific cyclin |
| UniProt Gene Name: | CCNE2 |
| UniProt Entry Name: | CCNE2_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using Cyclin E2 antibody (CAB7032). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of MCF7 cells using Cyclin E2 antibody (CAB7032). |
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